pSB1A3 lac
BBa_K627015 Version 1 (Component)
Plasmid backbone pSB1A3 carrying the lac operon induction system

BBa_K592201
BBa_K592201 Version 1 (Component)
Low to medium copy Lambda Red recombineering compatible plasmid

K592202
BBa_K592202 Version 1 (Component)
Low to medium copy Lambda Red recombineering compatible plasmid

BBa_K611018
BBa_K611018 Version 1 (Component)
Mutant Regulatory protein/promoter characterization plasmid

BBa_K654036
BBa_K654036 Version 1 (Component)
Anderson 1.0 Promoter Controlled Renilla Luciferase

BBa_K654037
BBa_K654037 Version 1 (Component)
Synechocystis psbA2 Promoter Controlled Renilla Luciferase

BBa_K654038
BBa_K654038 Version 1 (Component)
Synechocystis sigA promoter controlled Renilla Luciferase

BBa_K654039
BBa_K654039 Version 1 (Component)
Anderson 1.0 Promoter Controlled Firefly Luciferase

BBa_K654040
BBa_K654040 Version 1 (Component)
Synechocystis psbA2 controlled Firefly Luciferase

BBa_K654041
BBa_K654041 Version 1 (Component)
Synechocystis sigA promoter controlled Firefly Luciferase

BBa_K654047
BBa_K654047 Version 1 (Component)
Promoter Control Anderson E.coli 1.0 Reference Dual Luciferase Assay

BBa_K654050
BBa_K654050 Version 1 (Component)
Promoter Control psbA2 Reference Dual Luciferase Assay

BBa_K654053
BBa_K654053 Version 1 (Component)
Promoter Control sigA Reference Dual Luciferase Assay

BBa_K654070
BBa_K654070 Version 1 (Component)
psbA2 controlled Thioesterase

BBa_K654071
BBa_K654071 Version 1 (Component)
psbA2 controlled Fatty Acyl-CoA Synthetase (Ligase)

BBa_K654073
BBa_K654073 Version 1 (Component)
psbA2 controlled Wax Ester Synthase

BBa_K640004
BBa_K640004 Version 1 (Component)
Device for conjugation of plasmids from E. coli to pseudomonas

BBa_K640005
BBa_K640005 Version 1 (Component)
Device for conjugating plasmids from E. coli to pseudomonas

BBa_K654074
BBa_K654074 Version 1 (Component)
psbA2 controlled Fatty ACP Reductase

BBa_K896000
BBa_K896000 Version 1 (Component)
SQR(sulfide quinone reductase),from Synechococcus sp. PCC 7002 plasmid pAQ7

BBa_J72159
BBa_J72159 Version 1 (Component)
rbs.repA from ColE2-P9 plasmid

BBa_J72160
BBa_J72160 Version 1 (Component)
rbs2.repA from ColE2-P9 plasmid

BBa_K812000
BBa_K812000 Version 1 (Component)
Biobricked pCS2+ plasmid for frogs and chicken

BBa_K812001
BBa_K812001 Version 1 (Component)
Biobricked pSC2+ plasmid with pElastase promoter for frog use

BBa_K737035
BBa_K737035 Version 1 (Component)
Spot42 generator controlled by IPTG

BBa_K737036
BBa_K737036 Version 1 (Component)
Spot42 generator controlled by aTc

BBa_K737038
BBa_K737038 Version 1 (Component)
galK::GFP generator ligated to sRNA device controlled by IPTG

BBa_K737039
BBa_K737039 Version 1 (Component)
galK::GFP generator ligated to sRNA device controlled by aTc

BBa_K777101
BBa_K777101 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23100

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

BBa_K777102
BBa_K777102 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23104

BBa_K777103
BBa_K777103 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23105

BBa_K777104
BBa_K777104 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23106

BBa_K777105
BBa_K777105 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23109

BBa_K777106
BBa_K777106 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23112

BBa_K777107
BBa_K777107 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23113

BBa_K777108
BBa_K777108 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23114

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

mRFP1
BBa_I13502 Version 1 (Component)
Screening plasmid intermediate

OriTR
BBa_J01003 Version 1 (Component)
OriT-R (Origin of transfer for the R-plasmid nic region)

BBa_K142046
BBa_K142046 Version 1 (Component)
tet-controlled LacI generator

BBa_K142016
BBa_K142016 Version 1 (Component)
tet-controlled lacI IS mutant (R197A) generator

BBa_K142017
BBa_K142017 Version 1 (Component)
tet-controlled lacI IS mutant (R197F) generator

BBa_K116510
BBa_K116510 Version 1 (Component)
GFP under control of pOmpf promoter(K116500+E0240)

BBa_K142018
BBa_K142018 Version 1 (Component)
tet-controlled lacI IS mutant (T276A) generator

BBa_K142019
BBa_K142019 Version 1 (Component)
tet-controlled lacI IS mutant (T276F) generator

BBa_K142020
BBa_K142020 Version 1 (Component)
tet-controlled lacI IS mutant (R197A, T276A) generator

BBa_K142021
BBa_K142021 Version 1 (Component)
tet-controlled lacI IS mutant (R197A, T276F) generator

BBa_K112245
BBa_K112245 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!} inserted into K112995 plasmid