BBa_K223011BBa_K223011 Version 1 (Component)RBS + SoxR + TT + SoxS + RBS + BLH + RBS + RALDH II + TT
BBa_K1022122BBa_K1022122 Version 1 (Component)pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Magainin II : RBS TetR :TT
BBa_M36732BBa_M36732 Version 1 (Component)EfeU iron (II) transporter + 5' UTR (medium) + terminator + Fur promoter + Gemini
E coil ferBBa_K1189037 Version 1 (Component)His tag + Human ferritin di-subunit with E coil under LacI promoter
BBa_K1159312BBa_K1159312 Version 1 (Component)N-terminal <i>Strep</i>-tag-II in RFC[25] N-Part
BBa_I714004BBa_I714004 Version 1 (Component)R100 relaxase ( nick the oriT of the R100 IncF-II conjugative plasmid )
BBa_K1159014BBa_K1159014 Version 1 (Component)Membrane-anchored Erythromycin Esterase Type II in RFC[25] N-Part
BBa_M36983BBa_M36983 Version 1 (Component)Type II Secreted Antifreeze Actuator in E. coli (5-Repeat) Correct
BBa_M36982BBa_M36982 Version 1 (Component)Type II Secreted Antifreeze Actuator in E. coli (1-Repeat) Correct
BSMT2BBa_J45007 Version 1 (Component)SAM:benzoic acid/salicylic acid carboxyl methyltransferase II; converts salicylic acid to methyl sal
BBa_K223020BBa_K223020 Version 1 (Component)WCP + RBS + SoxR + TT + SoxS + RBS + BLH + RBS + RALDH II + TT
BBa_K223019BBa_K223019 Version 1 (Component)MCP + RBS + SoxR + TT + SoxS + RBS + BLH + RBS + RALDH II + TT
BBa_K223018BBa_K223018 Version 1 (Component)SCP + RBS + SoxR + TT + SoxS + RBS + BLH + RBS + RALDH II + TT
BBa_J06905BBa_J06905 Version 1 (Component)Lambda cI857 (Lambda II) QPI regulated by 434 cI with mCherry
BBa_J06915BBa_J06915 Version 1 (Component)Lambda cI857 (Lambda II) QPI regulated by 434 cI with EYFP
BBa_J06965BBa_J06965 Version 1 (Component)434 cI/lambda cI857 (Lambda II) RS flip-flop with EYFP
BBa_K1159005BBa_K1159005 Version 1 (Component)Secretory Erythromycin Esterase Type II (IgKappa-SigP_EreB) in RFC[25] N-Part
BBa_K1351036BBa_K1351036 Version 1 (Component)Quorum sensing two component system (AIP-II sensing histidine kinase agrC, response regulator agrA)
BBa_K2050419BBa_K2050419 Version 1 (Component)RNA Polimerase III Promoter (H1 promoter)
BBa_K223050BBa_K223050 Version 1 (Component)SoxR - SoxS System with Blh and RALDH II system and Lac promoter
BBa_J06574BBa_J06574 Version 1 (Component)Construction intermediate: cI857 (Lamda II) with RBS and forward terminator (B0034.J06503.B0014)
TALEA-difeBBa_K1189021 Version 1 (Component)TALE-A ferritin di-subunit direct fusion w/ hisTag under lacI promoter, and linker
BBa_K1022126BBa_K1022126 Version 1 (Component)pT7: RBS: His6- SUMO: Magainin II: RBS: TetR:TT : pTet: cI: TT : pcI: Ulp : Lysis
BBa_M31786BBa_M31786 Version 1 (Component)1st half of Gene III- preBamHI cut
BBa_K1159309BBa_K1159309 Version 1 (Component)19 AA Linker with N-terminal <i>Strep</i>-tag II and C-terminal TEV cleavage site in RFC[25]
BBa_K2050422BBa_K2050422 Version 1 (Component)Mouse H1 promoter (RNA Polymerase III promoter)
BBa_K887003BBa_K887003 Version 1 (Component)Plac+B0034+alsS+zif268+B0034+PBS II+ilvC+B0034+HIVC+ilvD+Ptet+B0032+kivD+B0015
luxR-ndhBBa_K1036000 Version 1 (Component)lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II
BBa_K1453304BBa_K1453304 Version 1 (Component)pBluescript II KS(+)_5_copy
BBa_K1453303BBa_K1453303 Version 1 (Component)pBluescript II KS(+)_3_copy
Strep-TagBBa_I757014 Version 1 (Component)Strep-Tag II affinity tag
BBa_M31114BBa_M31114 Version 1 (Component)M13, gene II from HpaI to the end [1-831bp]
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.