BBa_K908015
BBa_K908015 Version 1 (Component)
Microcin B17, Gene A flanked by rbs and Attc sequence

BBa_K908016
BBa_K908016 Version 1 (Component)
Microcin B17, Gene B flanked by Attc sequence and rbs

BBa_K908017
BBa_K908017 Version 1 (Component)
Microcin B17, Gene C flanked by Attc sequence and rbs

BBa_K908018
BBa_K908018 Version 1 (Component)
Microcin B17, Gene F flanked by Attc sequence and rbs

BBa_K908019
BBa_K908019 Version 1 (Component)
Microcin C7, Gene E flanked by Attc sequence and rbs

BBa_K908020
BBa_K908020 Version 1 (Component)
Microcin C7, Gene C flanked by Attc sequence and rbs

BBa_K908021
BBa_K908021 Version 1 (Component)
Microcin C7, Gene F flanked by Attc sequence and rbs

BBa_K883151
BBa_K883151 Version 1 (Component)
CCMV coat protein gene 25AA N-terminal replaced with HIStag under IPTG inducable promotor

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_J24815
BBa_J24815 Version 1 (Component)
J23101 promoter with Renilla gene attached

BBa_J24816
BBa_J24816 Version 1 (Component)
J23101 promoter with Luc gene attached

T7Gene6
BBa_K093001 Version 1 (Component)
T7 Gene 6 exonuclease

mtrB
BBa_K098989 Version 1 (Component)
mtrB coding region

BBa_K098997
BBa_K098997 Version 1 (Component)
cIts coding region

BBa_K098998
BBa_K098998 Version 1 (Component)
cIts coding region with rbs

BBa_K143067
BBa_K143067 Version 1 (Component)
AmyE integratable PoPS generator (P43-gsiB)

BBa_K143068
BBa_K143068 Version 1 (Component)
AmyE integratable PoPS generator (P43-spoVG)

BBa_K143069
BBa_K143069 Version 1 (Component)
AmyE integratable PoPS generator (Pveg-gsiB)

BBa_K143070
BBa_K143070 Version 1 (Component)
AmyE integratable PoPS generator (Pveg-spoVG)

BBa_K143071
BBa_K143071 Version 1 (Component)
AmyE integratable PoPS generator (P43-gsiB) (with CmR)

BBa_K143072
BBa_K143072 Version 1 (Component)
AmyE integratable PoPS generator (P43-spoVG) (with CmR)

BBa_K143073
BBa_K143073 Version 1 (Component)
AmyE integratable PoPS generator (Pveg-gsiB) (with CmR)

BBa_K143074
BBa_K143074 Version 1 (Component)
AmyE integratable PoPS generator (Pveg-spoVG) (with CmR)

BBa_K090503
BBa_K090503 Version 1 (Component)
Gram-Positive General Constitutive Promoter

BBa_K082037
BBa_K082037 Version 1 (Component)
RhlI & GFP generator

BBa_K082035
BBa_K082035 Version 1 (Component)
RhlI generator

BBa_K082034
BBa_K082034 Version 1 (Component)
lacI regulated GFP generator

BBa_K082030
BBa_K082030 Version 1 (Component)
luxI generator on PSB3C5

BBa_K082029
BBa_K082029 Version 1 (Component)
LuxI generator

BBa_I0466
BBa_I0466 Version 1 (Component)
RhlR Protein Generator

BBa_K082021
BBa_K082021 Version 1 (Component)
RhlI generator

BBa_K082020
BBa_K082020 Version 1 (Component)
LuxR generator

BBa_K082019
BBa_K082019 Version 1 (Component)
GFP generator

BBa_K082017
BBa_K082017 Version 1 (Component)
general recombine system

BBa_K082036
BBa_K082036 Version 1 (Component)
LuxR & RhlI generator

BBa_K156027
BBa_K156027 Version 1 (Component)
Genetic attenuator (synthetic transcription terminator) ~68% efficient

BBa_K142206
BBa_K142206 Version 1 (Component)
strong constitutive T4 ligase generator

BBa_K142207
BBa_K142207 Version 1 (Component)
medium constitutive T4 ligase generator

BBa_K142046
BBa_K142046 Version 1 (Component)
tet-controlled LacI generator

BBa_K142016
BBa_K142016 Version 1 (Component)
tet-controlled lacI IS mutant (R197A) generator

BBa_K142017
BBa_K142017 Version 1 (Component)
tet-controlled lacI IS mutant (R197F) generator

BBa_K142018
BBa_K142018 Version 1 (Component)
tet-controlled lacI IS mutant (T276A) generator

BBa_K142019
BBa_K142019 Version 1 (Component)
tet-controlled lacI IS mutant (T276F) generator

BBa_K142020
BBa_K142020 Version 1 (Component)
tet-controlled lacI IS mutant (R197A, T276A) generator

BBa_K142021
BBa_K142021 Version 1 (Component)
tet-controlled lacI IS mutant (R197A, T276F) generator

BBa_K142022
BBa_K142022 Version 1 (Component)
tet-controlled lacI IS mutant (R197F, T276A) generator

BBa_K142023
BBa_K142023 Version 1 (Component)
tet-controlled lacI IS mutant (R197F, T276F) generator