BBa_K157002
BBa_K157002 Version 1 (Component)
Transmembrane region of the EGF-Receptor (ErbB-1)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K102911
BBa_K102911 Version 1 (Component)
TA13 gate from synthetic algorithm v1.2

Q04510+RFP
BBa_K131018 Version 1 (Component)
Intermediate for the Response circuit

BBa_K125810
BBa_K125810 Version 1 (Component)
slr2016 signal sequence + GFP fusion for secretion of GFP

BBa_K156009
BBa_K156009 Version 1 (Component)
OFP (orange fluorescent protein)

BBa_K102905
BBa_K102905 Version 1 (Component)
TA7 gate from synthetic algorithm v1.1

lexA
BBa_K165020 Version 1 (Component)
LexA Synthetic Activator (untagged)

BBa_K165021
BBa_K165021 Version 1 (Component)
Kozak + LexA Synthetic Activator (untagged)

BBa_K165022
BBa_K165022 Version 1 (Component)
Zif268-HIV synthetic activator (untagged)

BBa_K165023
BBa_K165023 Version 1 (Component)
Kozak + Zif268-HIV synthetic activator (untagged)

OriTR
BBa_J01003 Version 1 (Component)
OriT-R (Origin of transfer for the R-plasmid nic region)

BBa_K156024
BBa_K156024 Version 1 (Component)
RBS + OFP

BBa_K116510
BBa_K116510 Version 1 (Component)
GFP under control of pOmpf promoter(K116500+E0240)

BBa_S04142
BBa_S04142 Version 1 (Component)
The Partial Butanol Operon (Parts I725021+I725022)

BBa_S04143
BBa_S04143 Version 1 (Component)
The Partial Butanol Operon (Parts I725023+I725024)

BBa_S04144
BBa_S04144 Version 1 (Component)
The Partial Butanol Operon (Parts I725024+I725025)

BBa_S04145
BBa_S04145 Version 1 (Component)
The Partial Butanol Operon (Parts I725021+I725022+I725023)

BBa_S04147
BBa_S04147 Version 1 (Component)
The Partial Butanol Operon (5 inserts from I725021 to I725025)

BBa_K156027
BBa_K156027 Version 1 (Component)
Genetic attenuator (synthetic transcription terminator) ~68% efficient

BBa_K142024
BBa_K142024 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A) and TetR expression cassette

BBa_K142025
BBa_K142025 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F) and TetR expression cassette

BBa_K142026
BBa_K142026 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (T276A) and TetR expression cassette

BBa_K142027
BBa_K142027 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (T276F) and TetR expression cassette

BBa_K142028
BBa_K142028 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276A)/TetR expression cassette

BBa_K142029
BBa_K142029 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276F)/TetR expression cassette

BBa_K142030
BBa_K142030 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F, T276A)/TetR expression cassette

BBa_K142031
BBa_K142031 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F, T276F)/TetR expression cassette

BBa_K116501
BBa_K116501 Version 1 (Component)
RpaA(regulator of phycobilisome-associated)

BBa_K116503
BBa_K116503 Version 1 (Component)
GFP under control of pOmpC promoter.R0082+E0240

BBa_K156028
BBa_K156028 Version 1 (Component)
Genetic attenuator (synthetic transciption terminator) ~45% efficient

BBa_K156029
BBa_K156029 Version 1 (Component)
Genetic attenuator (synthetic transcription terminator) ~17% efficient

BBa_K116520
BBa_K116520 Version 1 (Component)
GFP under control of RpaA activated pOmpF promoter

BBa_K116523
BBa_K116523 Version 1 (Component)
GFP under control of RpaA activated pOmpC promoter

BBa_K116000
BBa_K116000 Version 1 (Component)
A homolog of OCT3 (organic cation transporter 3) from E coli. K12

BBa_K116002
BBa_K116002 Version 1 (Component)
measurement of NhaA promoter activity

BBa_K079051
BBa_K079051 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator

BBa_K079052
BBa_K079052 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato

BBa_K153008
BBa_K153008 Version 1 (Component)
CqsA - the CAI-1 producer

BBa_K089005
BBa_K089005 Version 1 (Component)
-35 to Tc start site of phaC

BBa_K089006
BBa_K089006 Version 1 (Component)
-663 to Tc start site of phaC

BBa_K156035
BBa_K156035 Version 1 (Component)
Promoter + RBS + OFP

BBa_K112125
BBa_K112125 Version 1 (Component)
OriV origin of replication

BBa_K165079
BBa_K165079 Version 1 (Component)
pGAL1 + Null synthetic activator on pRS305

BBa_K152004
BBa_K152004 Version 1 (Component)
Promoterless Synthetic Operon of CrtE,B,I,Y

BBa_K152005
BBa_K152005 Version 1 (Component)
Promoterless Synthetic Operon of CrtE,B,I,Y and GFP

BBa_K152006
BBa_K152006 Version 1 (Component)
frdBCD operon from E. coli (missing the first gene fumarate oxidase)

BBa_K152007
BBa_K152007 Version 1 (Component)
Synthetic operon of CrtEBIY and FrdBCD and GFP

BBa_K152008
BBa_K152008 Version 1 (Component)
250 bp fragment from the Zea mays gene TB1

BBa_J70031
BBa_J70031 Version 1 (Component)
Linker for BioScaffold Parts, example of Gamma BioScaffold part