BBa_K750001
BBa_K750001 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 1.0 strength)

BBa_K750002
BBa_K750002 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.6 strength)

BBa_K750003
BBa_K750003 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.3 strength)

BBa_K750004
BBa_K750004 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.07 strength)

BBa_K817015
BBa_K817015 Version 1 (Component)
srnBC toxin-antitoxin cassette

BBa_K737000
BBa_K737000 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K737001
BBa_K737001 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K758004
BBa_K758004 Version 1 (Component)
GAL4, this part coding protein binds and activates the UAS sequence

BBa_K817050
BBa_K817050 Version 1 (Component)
Novel thermal promoter, Phs

UAS-LacZ
BBa_K758005 Version 1 (Component)
UAS LacZ, this part expresses LacZ in the presence of GAL4

UAS-EGFP
BBa_K758006 Version 1 (Component)
UAS EGFP, this part expresses EGFP in the presence of GAL4 protein.

BBa_K812012
BBa_K812012 Version 1 (Component)
OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin

AckA
BBa_K737002 Version 1 (Component)
This coding is the acetate kinase.

BBa_K863010
BBa_K863010 Version 1 (Component)
tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag

BBa_K750005
BBa_K750005 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.01 strength)

CBDcex
BBa_K863101 Version 1 (Component)
Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)

CBDclos
BBa_K863111 Version 1 (Component)
Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)

BBa_K739003
BBa_K739003 Version 1 (Component)
promoter of raxRH + RBS + mRFP

BBa_K873003
BBa_K873003 Version 1 (Component)
conjugative transfer of RFG

BBa_K863011
BBa_K863011 Version 1 (Component)
tthl laccase from Thermus thermophilus

BBa_K737058
BBa_K737058 Version 1 (Component)
Spot42 is a multitarget small RNA that mediates the discoordinate expression of the E.coli galactose

BBa_K737033
BBa_K737033 Version 1 (Component)
galK is the leader sequence of galactokinase (GalK)

BBa_K863012
BBa_K863012 Version 1 (Component)
tthl laccase from T. thermophilus with constitutive promoter J23100, RBS and His-tag

BBa_K737048
BBa_K737048 Version 1 (Component)
The leading sequence involved in the nanC transcript which can act as the target of the sRNA, spot42

BBa_K737049
BBa_K737049 Version 1 (Component)
The leading sequence involved in the YtfJ transcript which can act as the target of the sRNA, spot42

BBa_K737050
BBa_K737050 Version 1 (Component)
The leading sequence involved in the SrlA transcript which can act as the target of the sRNA, spot42

P-alsT
BBa_K818300 Version 1 (Component)
Promoter alsT, repressed by TnrA during conversion of NH4 to Glutamine in B. subtilis.

BBa_K769002
BBa_K769002 Version 1 (Component)
Kinase domain of EnvZ

CBDcex(T7)
BBa_K863102 Version 1 (Component)
Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)

BBa_K777101
BBa_K777101 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23100

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

BBa_K777102
BBa_K777102 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23104

BBa_K777103
BBa_K777103 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23105

BBa_K777104
BBa_K777104 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23106

BBa_K777105
BBa_K777105 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23109

BBa_K777106
BBa_K777106 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23112

BBa_K777107
BBa_K777107 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23113

BBa_K777108
BBa_K777108 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23114

pLac ColE2
BBa_K914001 Version 1 (Component)
pLac Colicin E2 Immunity protein

BBa_K914002
BBa_K914002 Version 1 (Component)
repressilator RBS Colicin E2 Immunity protein

BBa_K098984
BBa_K098984 Version 1 (Component)
lac inducible mtrB (high expression of QPI)

BBa_K098983
BBa_K098983 Version 1 (Component)
lac inducible mtrB (low expression of QPI)

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

Cello E. Coli Parts
Eco1C1G1T1_collection Version 1 (Collection)
These are the Cello parts for E. Coli circuits

BBa_K102906
BBa_K102906 Version 1 (Component)
TA8 gate from synthetic algorithm v1.1

BBa_K102907
BBa_K102907 Version 1 (Component)
TA9 gate from synthetic algorithm v1.1

BBa_K102908
BBa_K102908 Version 1 (Component)
TA10 gate from synthetic algorithm v1.1

BBa_K102909
BBa_K102909 Version 1 (Component)
TA11 gate from synthetic algorithm v1.1

BBa_K102910
BBa_K102910 Version 1 (Component)
TA12 gate from synthetic algorithm v1.1