BBa_K769016
BBa_K769016 Version 1 (Component)
Promoter(high)-RBS-lux CABFEG-DT

BBa_K769017
BBa_K769017 Version 1 (Component)
Promoter(Pbad)-RBS-luxA-DT-Promoter(high)-RBS-lux CDBFEG-DT

BBa_K769018
BBa_K769018 Version 1 (Component)
Promoter(Pbad)-RBS-luxD-DT-Promoter(high)-RBS-lux CABFEG-DT

BBa_K782001
BBa_K782001 Version 1 (Component)
2x[NicTAL]+2x[TALD] operator_CMV promoter_mCitrine

P-alsT
BBa_K818300 Version 1 (Component)
Promoter alsT, repressed by TnrA during conversion of NH4 to Glutamine in B. subtilis.

BBa_K782002
BBa_K782002 Version 1 (Component)
4x[NicTAL]+4x[TALD] operator_CMV promoter_mCitrine

BBa_K782003
BBa_K782003 Version 1 (Component)
1x[NicTAL]+1x[TALD] operator_CMV promoter_mCitrine

BBa_K777102
BBa_K777102 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23104

BBa_K782014
BBa_K782014 Version 1 (Component)
12x[TALD] operator_CMV promoter_mCitrine

BBa_K782015
BBa_K782015 Version 1 (Component)
12x[NicTAL] operator_CMV promoter_mCitrine

BBa_K777103
BBa_K777103 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23105

BBa_K863020
BBa_K863020 Version 1 (Component)
bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag

BBa_K777104
BBa_K777104 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23106

BBa_K777105
BBa_K777105 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23109

BBa_K777106
BBa_K777106 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23112

BBa_K777107
BBa_K777107 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23113

BBa_K777108
BBa_K777108 Version 1 (Component)
flhDC operon under the control of constitutive promoter J23114

BM-CFP
BBa_K774004 Version 1 (Component)
Bacterial-Mammalian promoter with eCFP reporter: BBaK216005 + CArG promoter sequence + BBa_E0420

BM-RFP
BBa_K774005 Version 1 (Component)
Bacterial-Mammalian promoter with RFP reporter: BBaK216005 + BHN + CArG promoter sequence E9-ns2 + B

MB-CFP
BBa_K774006 Version 1 (Component)
Mammalian-Bacterial promoter with eCFP reporter: CArG promoter sequence + BBaK216005 + BBa_E0420

MB-RFP
BBa_K774007 Version 1 (Component)
Mammalian-Bacterial promoter with RFP reporter: CArG promoter sequence + BBaK216005 + BBa_K08101

BBa_K777125
BBa_K777125 Version 1 (Component)
Constitutive J23100 promoter

BBa_K782016
BBa_K782016 Version 1 (Component)
10x[TALA+TALB] operator_CMV promoter_mCitrine

BBa_K782017
BBa_K782017 Version 1 (Component)
10x[TALA] operator_CMV promoter_mCitrine

BBa_K782018
BBa_K782018 Version 1 (Component)
10x[TALB] operator_CMV promoter_mCitrine

BBa_K782019
BBa_K782019 Version 1 (Component)
10x[TALA+TALB] operator_CMV promoter_fLuciferase

BBa_K782020
BBa_K782020 Version 1 (Component)
10x[TALA] operator_CMV promoter_fLuciferase

BBa_K782021
BBa_K782021 Version 1 (Component)
10x[TALB] operator_CMV promoter_fLuciferase

BBa_K782022
BBa_K782022 Version 1 (Component)
12x[TALD] operator_CMV promoter_fLuciferase

BBa_K782023
BBa_K782023 Version 1 (Component)
12x[NicTAL] operator_CMV promoter_fLuciferase

BBa_K782024
BBa_K782024 Version 1 (Component)
2x[NicTAL]+2x[TALD] operator_minimal promoter_mCitrine

BBa_K782025
BBa_K782025 Version 1 (Component)
4x[NicTAL]+4x[TALD] operator_minimal promoter_mCitrine

BBa_K782026
BBa_K782026 Version 1 (Component)
7x[TALA+TALB] operator_minimal promoter_mCitrine

BBa_K782027
BBa_K782027 Version 1 (Component)
7x[TALA] operator_minimal promoter_mCitrine

BBa_K782028
BBa_K782028 Version 1 (Component)
10x[TALA+TALB] operator_minimal promoter_mCitrine

BBa_K782029
BBa_K782029 Version 1 (Component)
10x[TALA] operator_minimal promoter_mCitrine

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K098984
BBa_K098984 Version 1 (Component)
lac inducible mtrB (high expression of QPI)

BBa_K098983
BBa_K098983 Version 1 (Component)
lac inducible mtrB (low expression of QPI)

BBa_K157002
BBa_K157002 Version 1 (Component)
Transmembrane region of the EGF-Receptor (ErbB-1)

BBa_K782030
BBa_K782030 Version 1 (Component)
10x[TALB] operator_minimal promoter_mCitrine

BBa_K782031
BBa_K782031 Version 1 (Component)
12x[TALD] operator_minimal promoter_mCitrine

BBa_K782032
BBa_K782032 Version 1 (Component)
10x[TALA+TALB] operator_minimal promoter_fLuciferase

BBa_K782033
BBa_K782033 Version 1 (Component)
10x[TALA] operator_minimal promoter_fLuciferase

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_S04136
BBa_S04136 Version 1 (Component)
terminator and cI promoter

BBa_K098995
BBa_K098995 Version 1 (Component)
heat sensitive cI QPI with high promoter

BBa_K098993
BBa_K098993 Version 1 (Component)
heat sensitive cI QPI with low promoter

BBa_K098991
BBa_K098991 Version 1 (Component)
cI promoter with GFP reporter