iGEM 2019 Plates
iGEM_2019_Plates Version 1 (Collection)
384-well plates of dried DNA distributed by iGEM in Spring 2019

iGEM 2018 Plates
iGEM_2018_Plates Version 1 (Collection)
384-well plates of dried DNA distributed by iGEM in Spring 2018

BBa_K086001
BBa_K086001 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086012
BBa_K086012 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP reporter

BBa_K086007
BBa_K086007 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP reporter

BBa_K086013
BBa_K086013 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K086004
BBa_K086004 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP promoter

BBa_K086014
BBa_K086014 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K086016
BBa_K086016 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K086011
BBa_K086011 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP reporter

BBa_K086003
BBa_K086003 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086010
BBa_K086010 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP reporter

BBa_K086015
BBa_K086015 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K2116068
BBa_K2116068 Version 1 (Component)
AND gate regulated by norR and esaR (internal esabox, overlapping with sigma 54 binding site, before

BBa_K1363003
BBa_K1363003 Version 1 (Component)
OriT-K592016 the blue sensor which can be transfered into other bacterias by S17-1

BBa_K831014
BBa_K831014 Version 1 (Component)
Inducible istR (inhibitor of SOS-induced toxicity by RNA) under the control of lac promoter

BBa_K1189029
BBa_K1189029 Version 1 (Component)
TALE-A with a his tag linked to a K coil under the control of a LacI promoter

BBa_K539691
BBa_K539691 Version 1 (Component)
promoter(lacI regulated)+Alss+ilvC+ilvD(each preceded by own RBS)and RNA thermometer+terminator

BBa_K833014
BBa_K833014 Version 1 (Component)
constitutive promoter followed by a lox site with a bidirectional terminator and then a lox site.

BBa_K658011
BBa_K658011 Version 1 (Component)
a bacteria population-control device with lux pR-3/5 driven by lacl+pL

BBa_K1706000
BBa_K1706000 Version 1 (Component)
A promoter activitated by formaldehyde

BBa_K1361004
BBa_K1361004 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag

BBa_K079052
BBa_K079052 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato

BBa_K079020
BBa_K079020 Version 1 (Component)
GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein

BBa_K079051
BBa_K079051 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator

BBa_K887004
BBa_K887004 Version 1 (Component)
Plac+alsS+ilvC+ilvD(each preceded by own zinc-finger and RBS)+Ptet+B0032+kivD+B0015

BBa_K987001
BBa_K987001 Version 1 (Component)
This is a composite part which has the function to invert the temperature activation by the part: BB

BBa_K843003
BBa_K843003 Version 1 (Component)
Lambda cI regulated by PLacI

BBa_K1361007
BBa_K1361007 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative

BBa_K886002
BBa_K886002 Version 1 (Component)
Recombination Device composed by Cre-lox71

BBa_K1616004
BBa_K1616004 Version 1 (Component)
VVD linked to YN155 (YFP Nter split)

PL GFP
BBa_K193000 Version 1 (Component)
GFP reporter regulated by CI.

pTetR-LacI
BBa_I763030 Version 1 (Component)
LacI coding device regulated by pTetR

BBa_K1321362
BBa_K1321362 Version 1 (Component)
sfGFP fused to CBDcex driven by LacI

BBa_I763003
BBa_I763003 Version 1 (Component)
GFP coding device switched on by IPTG

BBa_K1615108
BBa_K1615108 Version 1 (Component)
mRFP fused to CBDclos driven by LacI promoter

PLac-LacY-
BBa_I763013 Version 1 (Component)
LacY and cI coding device switched on by IPTG

BBa_K258003
BBa_K258003 Version 1 (Component)
Granulysin, a T Cell Product,Kills Bacteria by Altering Membrane Permeability

BBa_K395102
BBa_K395102 Version 1 (Component)
GFP reporter repressed by LuxR and 3OC6HSL (K395005:K121013)

BBa_K395103
BBa_K395103 Version 1 (Component)
GFP reporter repressed by LuxR and 3OC6HSL (K395006:K121013)

BBa_J58014
BBa_J58014 Version 1 (Component)
Promoter activated by OmpR-P with the reporter GFP

BBa_K290001
BBa_K290001 Version 1 (Component)
constitutive RhlR with bicistronic LuxI - GFP controlled by pRhl

BBa_J58015
BBa_J58015 Version 1 (Component)
Mutated promoter activated by OmpR-P with the reporter GFP

pCMV-ECFP-
BBa_I763023 Version 1 (Component)
LacI coding device with ECFP as a reporter regulated by pCMV

BBa_K300096
BBa_K300096 Version 1 (Component)
Double phasin and intein separed by a flexible protein domain linker

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K2144011
BBa_K2144011 Version 1 (Component)
Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter

BBa_K831011
BBa_K831011 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12