BBa_K309021
BBa_K309021 Version 1 (Component)
pStr-220::eCFP::3'UTR (for use in C. elegans)

BBa_K1418040
BBa_K1418040 Version 1 (Component)
CFP

BBa_K331040
BBa_K331040 Version 1 (Component)
N-terminal Arg Tagged Fluorescent Proteins (CFP/YFP)

BBa_K1707037
BBa_K1707037 Version 1 (Component)
cI repressor controlling the expression of CFP-ssRA

BBa_K331041
BBa_K331041 Version 1 (Component)
C-terminal Arg Tagged Fluorescent Proteins (YFP/CFP)

BBa_K331038
BBa_K331038 Version 1 (Component)
N-terminal Arg Tagged Fluorescent Proteins (YFP/CFP)

BBa_K323081
BBa_K323081 Version 1 (Component)
YFP link CFP under T7 promotor - exhibits FRET

BBa_K331039
BBa_K331039 Version 1 (Component)
C-terminal Arg Tagged Fluorescent Proteins (YFP/CFP)

BBa_I763021
BBa_I763021 Version 1 (Component)
LacI coding device with ECFP as a reporter regulated by pCMV

BBa_K561003
BBa_K561003 Version 1 (Component)
PfdhF+RFP+tetR+Pvgb+YFP+tetR+Ptet+CFP

BBa_K876040
BBa_K876040 Version 1 (Component)
TT-pLasR-LuxS-RBS-CFP-TT-pLuxR-TetR

BBa_K624051
BBa_K624051 Version 1 (Component)
pYMB essentials + RBS(trunc.) + LLO + RBS(trunc.) + Invasin + RBS(trunc.) + ECFP

BBa_K876058
BBa_K876058 Version 1 (Component)
pTetR-LasR-TT-pLasR-RBS-CFP-Term-Term

BBa_K142075
BBa_K142075 Version 1 (Component)
lac-controlled CFP generator with constitutive lacI expression cassette

BBa_K1707016
BBa_K1707016 Version 1 (Component)
TetR-ssRA repressor controlling the expression of CFP-ssRA

BBa_K1707029
BBa_K1707029 Version 1 (Component)
thermosensitive cI repressor controlling the expression of CFP-ssRA

BBa_K331018
BBa_K331018 Version 1 (Component)
Lumazine Synthase Microcompartment Test Construct (ECFP & EYFP with N-Terminal Arginine Tag)

BBa_K331019
BBa_K331019 Version 1 (Component)
Lumazine Synthase Microcompartment Test Construct (ECFP & EYFP with C-Terminal Arginine Tag)

BBa_K272991
BBa_K272991 Version 1 (Component)
Rbs-ccdB toxin - EYFP-constitutive promoter-Rbs-Streptomycin resistance marker-ECFP

BBa_K249025
BBa_K249025 Version 1 (Component)
Target C-terminal YFP and C-terminal CFP into Lumazine

BBa_K249024
BBa_K249024 Version 1 (Component)
Target N-terminal YFP and N-terminal CFP into Lumazine

BBa_K876036
BBa_K876036 Version 1 (Component)
pTetR-LasR-TT-pLasR-RBS-CFP-TT-pLuxR-TetR

BBa_K542008
BBa_K542008 Version 1 (Component)
pLacI Regulated Lumazine Synthase and pBAD Inverse-Regulated Arg-tagged ECFP and EYFP

BBa_K309032
BBa_K309032 Version 1 (Component)
pMec-7::eCFP::unc-54 3' UTR (for use in C. elegans chassis)

BBa_K1707005
BBa_K1707005 Version 1 (Component)
TetR repressor controlling the expression of CFP tagged with LVA

BBa_K309022
BBa_K309022 Version 1 (Component)
pOdr-10::eCFP::unc-54 3'UTR (for use in C. elegans chassis)

BBa_K1616003
BBa_K1616003 Version 1 (Component)
VVD link to YC155 (YFP Cter split)

BBa_K1616004
BBa_K1616004 Version 1 (Component)
VVD linked to YN155 (YFP Nter split)

BBa_I13990
BBa_I13990 Version 1 (Component)
Synchronator 2 w/ CFP under control of Lac (I4202.I13601)

BBa_K1960003
BBa_K1960003 Version 1 (Component)
Quorum sensing controller for gram-negative bacterium with cyan fluorescent protein (CFP)

BBa_C0510
BBa_C0510 Version 1 (Component)
Combined expession system of cfp and yfp downstream to bgl antiterminators

c-di-AMP
BBa_K1045002 Version 1 (Component)
CFP Reporter under control of the c-di-AMP-dependent YdaO Riboswitch

BBa_K1051359
BBa_K1051359 Version 1 (Component)
K1051304(cln3 promoter) + K1051053(K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 term

BBa_K1051355
BBa_K1051355 Version 1 (Component)
K1051300 (cln2 promoter) + K1051053(K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 ter

BBa_K1051358
BBa_K1051358 Version 1 (Component)
K1051303(clb6 promoter) + K1051053(K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 term

BBa_K1051357
BBa_K1051357 Version 1 (Component)
K1051302 (clb5 promoter) + K1051053(K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 ter

BBa_K1051356
BBa_K1051356 Version 1 (Component)
K1051301(clb2 promoter) + K1051053(K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 term

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

tar-tsr
BBa_J22010 Version 1 (Component)
Lac promoter-Tar Receptor-CFP reporter-Tet promoter-Tsr receptor-RFP reporter

pCMV-ECFP-
BBa_I763023 Version 1 (Component)
LacI coding device with ECFP as a reporter regulated by pCMV

BBa_K136035
BBa_K136035 Version 1 (Component)
rbs TetR - ECFP

BBa_I15021
BBa_I15021 Version 1 (Component)
CFP generator

BBa_K323075
BBa_K323075 Version 1 (Component)
ATG cYFP link HIVC

BBa_J22202
BBa_J22202 Version 1 (Component)
pLac-CFP

BBa_K204021
BBa_K204021 Version 1 (Component)
ptetR+CFP

BBa_J06103
BBa_J06103 Version 1 (Component)
tetR repressible ECFP reporter