Co-opBBa_K233002 Version 1 (Component)Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway
BBa_K233001BBa_K233001 Version 1 (Component)Snowdrift 1 , using TorA. A module to secrete B-galactosidase using the TAT-export Pathway
Hax3-2xNGBBa_K2075023 Version 1 (Component)TAL-effector Hax3-2xNG
BBa_K1974022BBa_K1974022 Version 1 (Component)T7Promoter+RBS+Sf1a+linker+snowdrop-lectin+linker+6X His-Tag
BBa_K1974021BBa_K1974021 Version 1 (Component)T7Promoter+RBS+Hv1a+linker+snowdrop-lectin+linker+6X His-Tag
BBa_K1974023BBa_K1974023 Version 1 (Component)T7Promoter+RBS+OAIP+linker+snowdrop-lectin+linker+6X His-Tag
BBa_I716015BBa_I716015 Version 1 (Component)RFP without start ATG
BBa_K1036003BBa_K1036003 Version 1 (Component)lux pL controlled luxR with lux pR controlled gfp (LVA-tag)
BBa_K1441012BBa_K1441012 Version 1 (Component)DNA ligase from Escherichia coli with His-tag In pGAPz alpha A
BBa_K323075BBa_K323075 Version 1 (Component)ATG cYFP link HIVC
BBa_K1974033BBa_K1974033 Version 1 (Component)T7 Promoter+RBS+Hv1a+GS linker+snowdrop-lectin+linker+6X His-Tag
BBa_K2144011BBa_K2144011 Version 1 (Component)Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter
BBa_K1106013BBa_K1106013 Version 1 (Component)pag activator from PSP3 phage generator
BBa_K2172009BBa_K2172009 Version 1 (Component)Tac Promoter-RBS-GST-Thrombin Protease-GFP-Terminator
BBa_M36556BBa_M36556 Version 1 (Component)5' Bicistronic UTR (medium), does not include ATG start
BBa_K2123112BBa_K2123112 Version 1 (Component)Tac promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)
BBa_K2123115BBa_K2123115 Version 1 (Component)Universal promoter (Tac + JK26) for both growth phase with downstream mer operator + K081014
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.