Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Swetha Srinivasan, Samit Watve, Mandar Phatak, Chinar Patil
Date created: 2009-07-09 11:00:00
Date modified: 2015-05-08 01:11:35
Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K233002_sequence (Version 1) |
Description
This is a module that contains the following components:1. A constituive promoter
2. RBS (ribosome binding site)
3. An export tag viz. torA
4. Lac Z gene (Gene for enzyme B-galactosidase)
5. A reporter gene viz. GFP
6. TERM
this is an eternal co-operator strain...
Notes
a suitable export tag was synthesizd and added in frame. the export tag was designed based on the twin arginine translocation (tat) pathway from E.coliSource
source- biobricks torA,lacZ, pLac - eschercihia coli GFP- aequoria victoria| Access | Instance | Definition |
| public public public public public public public | BBa_J23118 BBa_B0030 BBa_K233306 BBa_I732005 BBa_E0040 BBa_B0010 BBa_B0012 | BBa_J23118 BBa_B0030 BBa_K233306 LacZ GFP BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23118 BBa_B0030 BBa_K233306 BBa_I732005 BBa_E0040 BBa_B0010 BBa_B0012 | 1,35 44,58 65,187 194,3268 3275,3994 4003,4082 4091,4131 | BBa_J23118 BBa_B0030 BBa_K233306 LacZ GFP BBa_B0010 BBa_B0012 |