Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Swetha Srinivasan, Samit Watve, Mandar Phatak, Chinar Patil
Date created: 2009-07-09 11:00:00
Date modified: 2015-05-08 01:11:35
Snowdrift 1 , using TorA. A module to secrete B-galactosidase using the TAT-export Pathway
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K233001_sequence (Version 1) |
Description
this is a module that contains the following components1.an inducible lac promoter with the operator( lacI binding sites)
2. RBS (ribosome binding site)
3. an export tag viz. torA
4. beta-galactosidase gene
5. a reporter gene viz. GFP
6. TERM
Notes
a suitable export tag was synthesizd and added in frame.the export tag was designed based on the twin arginine translocation (tat) pathway from E.coli
Source
source- biobrickstorA,lacZ, pLac - eschercihia coli
GFP- aequoria victoria
| Access | Instance | Definition |
| public public public public public public public | BBa_R0010 BBa_B0030 BBa_K233307 BBa_I732005 BBa_E0040 BBa_B0010 BBa_B0012 | LacI BBa_B0030 BBa_K233307 LacZ GFP BBa_B0010 BBa_B0012 |