BBa_K923004
BBa_K923004 Version 1 (Component)
T7 Terminator from pET14 Plasmid

BBa_K1044007
BBa_K1044007 Version 1 (Component)
T7 promoter (strong promoter from T7 bacteriophage)+RBS (Elowitz 1999) -- defines RBS efficiency

BBa_K133039
BBa_K133039 Version 1 (Component)
T7-CF-UREB-RGD-Histop

BBa_I746911
BBa_I746911 Version 1 (Component)
construction intermediate: T7 promoter - RBS

BBa_K1587005
BBa_K1587005 Version 1 (Component)
Butyrate synthesis pathway (BBa_1587004) without ccr gene regulated by constitutive promoter p(Bla)

BBa_K228099
BBa_K228099 Version 1 (Component)
T7 promoter+strong RBS+GFP

BBa_K1165012
BBa_K1165012 Version 1 (Component)
Ethylene Synthesis Cassette (SAM Synthetase, ACC Oxidase, ACC Synthase) under the Control of pTet Pr

BBa_K2005051
BBa_K2005051 Version 1 (Component)
mCherry with T7 expression (oxidation-resistant)

BBa_K1172306
BBa_K1172306 Version 1 (Component)
Riboflavin synthesis gene cluster from s. oneidensis under control of a strong Anderson promoter

BBa_K1172305
BBa_K1172305 Version 1 (Component)
Riboflavin synthesis gene cluster from s. oneidensis under control of a medium Anderson promoter

BBa_J44015
BBa_J44015 Version 1 (Component)
T7 promoter - RBS - RFP1 - hixC - RFP2

BBa_K1124107
BBa_K1124107 Version 1 (Component)
pLac-hpaBC-plambda-sRNA(anti-tyrR)-plambda-sRNA (anti-csrA) (L-DOPA synthesis device)

BBa_K1195011
BBa_K1195011 Version 1 (Component)
T7 Bacteriophage Minor Capsid Protein (Small Mutant 2)

BBa_K1195012
BBa_K1195012 Version 1 (Component)
T7 Bacteriophage Minor Capsid Protein (Large Mutant 2)

BBa_K258012
BBa_K258012 Version 1 (Component)
AI2-dependent KGF synthesis with RFP reporter and AHL production for Quaroum Sensin Death Mechanism

BBa_K145105
BBa_K145105 Version 1 (Component)
cI under T7 and P_R dual promotor

BBa_K1974013
BBa_K1974013 Version 1 (Component)
T7 Promoter+RBS+OAIP+linker+6X His-Tag

BBa_K145103
BBa_K145103 Version 1 (Component)
T7 with UmuD derived tag under constitutive promotor + RiboLock3d

BBa_K809710
BBa_K809710 Version 1 (Component)
GAL promoter + kozak + ZIM17 + T7 RNAP + ADH1 terminator

iGEM Parts Registry
igem_collection Version 1 (Collection)
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.

BBa_K145112
BBa_K145112 Version 1 (Component)
cI under T7 and PR_R dual promotor

BBa_K1497014
BBa_K1497014 Version 1 (Component)
Pelargonidin producing operon (low yield)- T7-B0034-F3H-B0034-DFR-B0034-ANS

BBa_K726009
BBa_K726009 Version 1 (Component)
T7 driven lac operated inducer for the rhl quorum-sensing system

BBa_K1974033
BBa_K1974033 Version 1 (Component)
T7 Promoter+RBS+Hv1a+GS linker+snowdrop-lectin+linker+6X His-Tag

BBa_K2144011
BBa_K2144011 Version 1 (Component)
Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.