BBa_K2172012
BBa_K2172012 Version 1 (Component)
Tac Promoter-RBS-GST-Thrombin Protease-SmCPS1-TEV-GFP-Terminator

BBa_I733006
BBa_I733006 Version 1 (Component)
Output Cells detect the light input and produce HSL

BBa_K539392
BBa_K539392 Version 1 (Component)
Vio operon ABDE(each preceded by their own ribosome binding site) with a terminator

BBa_K1334020
BBa_K1334020 Version 1 (Component)
a signal complex to test whether the lux system works

BBa_I715076
BBa_I715076 Version 1 (Component)
pLac promoter upstream of the Ec86 retron msr/msd sequence

BBa_I715075
BBa_I715075 Version 1 (Component)
pLac promoter upstream of the Ec83 retron msr/msd sequence

BBa_K1510119
BBa_K1510119 Version 1 (Component)
A truncated INPNC anchor protein that binds fragments on the cell surface

BBa_K1329002
BBa_K1329002 Version 1 (Component)
Insertion of the Metallothionein Cup-1-1 domain into Bacillus subtilis flagellin

BBa_K1165011
BBa_K1165011 Version 1 (Component)
AHL Receptor operon (LuxR, RhlR, CepR) under the Control of pOmpC Promoter

T7rnap
BBa_I716103 Version 1 (Component)
T7rnap (T7 RNA Polymerase) - ATG Start Codon

BBa_K1162005
BBa_K1162005 Version 1 (Component)
Scygonadin antimicrobial peptide from the horseshoe crab (<i>Scylla serrata</i>)

BBa_K1465208
BBa_K1465208 Version 1 (Component)
Carbonic anhydrase (csoS3) and shell-proteins (csoS14) of the carboxysome of H. neapolitanus

iGEM 2019 Distribution
iGEM2019Distribution_collection Version 1 (Collection)
Distribution of parts for the 2019 iGEM competition

iGEM 2018 Distribution
iGEM2018Distribution_collection Version 1 (Collection)
Distribution of parts for the 2018 iGEM competition

BBa_K323076
BBa_K323076 Version 1 (Component)
ATG cYFP link Blues link His stop T7 terminator

CMV + GFP
BBa_K1852000 Version 1 (Component)
GFP + CMV Promoter used for expression in oocyte cells. This part was planned to be used as a report

BBa_K1992005
BBa_K1992005 Version 1 (Component)
Tar native RBS expression system (promoter+RBS+coding+terminator)

BBa_K2100038
BBa_K2100038 Version 1 (Component)
pEXPR TRE - TALER21

BBa_K2100069
BBa_K2100069 Version 1 (Component)
pEXPR TRE: L7Ae

BBa_K2100075
BBa_K2100075 Version 1 (Component)
pEXPR TRE: mKate

BBa_K2100079
BBa_K2100079 Version 1 (Component)
pEXPR TRE: tagBFP

BBa_I733002
BBa_I733002 Version 1 (Component)
big toe meter

BBa_K2100037
BBa_K2100037 Version 1 (Component)
pEXPR TRE - TALER14

BBa_K2100036
BBa_K2100036 Version 1 (Component)
pEXPR TRE-BM3R1

KWR
BBa_K175030 Version 1 (Component)
Key for lock of weak (K175029) RBS (B0031) from the lock/key library TUD09

xis domain
BBa_K112203 Version 1 (Component)
{< xis >} The bacteriophage lambda xis gene without start or stop codons; assembly standard 21

BBa_K658010
BBa_K658010 Version 1 (Component)
a bacteria population-control device with lux pR-5 driven by lacl+pL

BBa_K658009
BBa_K658009 Version 1 (Component)
a bacteria population-control device with lux pR-3 driven by lacl+pL

BBa_K112210
BBa_K112210 Version 1 (Component)
{< int >} The integrase gene without start and stop codons, BBb format

BBa_K112214
BBa_K112214 Version 1 (Component)
{< ihfA!} The ihf alpha gene without a start codon, BBb format

BBa_K112215
BBa_K112215 Version 1 (Component)
{ihfA>} The ihf alpha gene without a stop codon, BBb format

BBa_K112220
BBa_K112220 Version 1 (Component)
{< ihfB!} The ihf beta gene without a start codon, BBb format

BBa_K112221
BBa_K112221 Version 1 (Component)
{ihfB>} The ihf beta gene without a stop codon, BBb format

BBa_K112207
BBa_K112207 Version 1 (Component)
{int!} The integrase gene with start and stop codon, BBb format

BBa_K199155
BBa_K199155 Version 1 (Component)
Measures the reverse activity of pLux in presence of pOmpC

BBa_K1412614
BBa_K1412614 Version 1 (Component)
Characterize the efficiency of promoter(<bbpart>BBa_K206000</bbpart>) with chemotaxis

BBa_K1412010
BBa_K1412010 Version 1 (Component)
Drive the E.coli engineered strain(CL-1) towards death region

BBa_K1412008
BBa_K1412008 Version 1 (Component)
Drive the E.coli engineered strain(CL-1) towards death region

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K180007
BBa_K180007 Version 1 (Component)
Game of Life - Secondary plasmid [tac pR, LuxI generator]

BBa_K1781004
BBa_K1781004 Version 1 (Component)
T18 - one of two fragments of the T25/T18 BACTH system

BBa_M45689
BBa_M45689 Version 1 (Component)
Constitutive promoter with a retinoic acid response element at the end

BBa_K363003
BBa_K363003 Version 1 (Component)
A calcium dependent response element binding site for the Crz1 activator

BBa_K363002
BBa_K363002 Version 1 (Component)
A calcium dependent response element binding site for the Crz1 activator

BBa_K363005
BBa_K363005 Version 1 (Component)
A calcium dependent response element binding site for the Crz1 activator

BBa_K363007
BBa_K363007 Version 1 (Component)
A calcium dependent response element binding site for the Crz1 activator

BBa_K363004
BBa_K363004 Version 1 (Component)
A calcium dependent response element binding site for the Crz1 activator

BBa_K112229
BBa_K112229 Version 1 (Component)
{a~pelB} The pelB leader ready to attach rbs, BBb format

BBa_K1465227
BBa_K1465227 Version 1 (Component)
sRNA of phosphofructokinase A under the control of T7 promoter (T7_sRNA:pfkA)

BBa_K112226
BBa_K112226 Version 1 (Component)
{a~ompT} The ompT gene ready to attach rbs, BBb site