BBa_K908019
BBa_K908019 Version 1 (Component)
Microcin C7, Gene E flanked by Attc sequence and rbs

BBa_K908020
BBa_K908020 Version 1 (Component)
Microcin C7, Gene C flanked by Attc sequence and rbs

BBa_K908021
BBa_K908021 Version 1 (Component)
Microcin C7, Gene F flanked by Attc sequence and rbs

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K098986
BBa_K098986 Version 1 (Component)
lac QPI driven by high promoter

BBa_K098985
BBa_K098985 Version 1 (Component)
lac QPI driven by low promoter

BBa_K098981
BBa_K098981 Version 1 (Component)
tet QPI driven by high promoter

BBa_K098980
BBa_K098980 Version 1 (Component)
tet QPI driven by low promoter

BBa_K086001
BBa_K086001 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086002
BBa_K086002 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP

BBa_K086003
BBa_K086003 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

SBFP2
BBa_K156010 Version 1 (Component)
SBFP2 (strongly enhanced blue fluorescent protein)

BBa_K116500
BBa_K116500 Version 1 (Component)
OmpF promoter that is activated or repressesed by OmpR according to osmolarity.

BBa_K116001
BBa_K116001 Version 1 (Component)
nhaA promoter, that can be regulated by pH and nhaR protein.

BBa_J04430
BBa_J04430 Version 1 (Component)
GFP coding device switched on by IPTG

pLac+GFP
BBa_I763004 Version 1 (Component)
GFP coding device switched on by IPTG

BBa_K142048
BBa_K142048 Version 1 (Component)
promotorless lacI expression cassette followed by constitutively active TetR generator

BBa_K079051
BBa_K079051 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator

BBa_K079052
BBa_K079052 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato

BBa_K086004
BBa_K086004 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP promoter

BBa_K086005
BBa_K086005 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP

BBa_K086006
BBa_K086006 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP

BBa_K086007
BBa_K086007 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP reporter

BBa_K086008
BBa_K086008 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP

BBa_K086009
BBa_K086009 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP

BBa_K086010
BBa_K086010 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP reporter

BBa_K086011
BBa_K086011 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP reporter

BBa_K086012
BBa_K086012 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP reporter

BBa_K086013
BBa_K086013 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K086016
BBa_K086016 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K086014
BBa_K086014 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K086015
BBa_K086015 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K127003
BBa_K127003 Version 1 (Component)
Polystyrene binding peptide inserted CPX generated by qurum sensing and constitutive GFP genarator

TrfA
BBa_K112129 Version 1 (Component)
TrfA with an RBS , followed by terminiator

xis/int
BBa_K112132 Version 1 (Component)
Xis Int controlled by temperature sensitive

BBa_K112147
BBa_K112147 Version 1 (Component)
lambda lysis device controlled by magnesium promoter

BBa_K112146
BBa_K112146 Version 1 (Component)
lambda lysis device controlled by PmgtCB magnesium promoter

BBa_J70305
BBa_J70305 Version 1 (Component)
GFP coding device switched on by IPTG (PsrI site removed)

RE
BBa_J3101 Version 1 (Component)
Recombinational Enhancer (RE) for Hin/Hix inverting

BBa_K239000
BBa_K239000 Version 1 (Component)
DegP promoter, activated by Phosporylated CpxR and SigmaE

BBa_K239001
BBa_K239001 Version 1 (Component)
Spy promoter, activated by Phosporylated CpxR, BaeR and Sigma70

BBa_K239002
BBa_K239002 Version 1 (Component)
HypB promoter, activated by Fnr during oxygen deficiency

BBa_K239003
BBa_K239003 Version 1 (Component)
Modified HypB promoter, activated by Fnr during oxygen deficiency

BBa_K239004
BBa_K239004 Version 1 (Component)
Part of narGP promoter, activated by Fnr during oxygen deficiency

BBa_K290000
BBa_K290000 Version 1 (Component)
constitutive RhlR with bicistronic LuxI - GFP controlled by pRhl

BBa_K290001
BBa_K290001 Version 1 (Component)
constitutive RhlR with bicistronic LuxI - GFP controlled by pRhl

BBa_K290002
BBa_K290002 Version 1 (Component)
constitutive LuxR, with bicistronic RhlI - EYFP controlled by lux pL

BBa_K290003
BBa_K290003 Version 1 (Component)
constitutive RhlR with bicistronic LuxI - ECFP controlled by pRhl

BBa_I763027
BBa_I763027 Version 1 (Component)
pLac activity Tester repressed by LacI