BBa_K861070
BBa_K861070 Version 1 (Component)
AdrA gene from E.coli K12

BBa_K861090
BBa_K861090 Version 1 (Component)
YhjH Gene From <i>E.coli str. K12</i>

BBa_K863030
BBa_K863030 Version 1 (Component)
tvel5 laccase cDNA sequence from Trametes versicolor

BBa_K863012
BBa_K863012 Version 1 (Component)
tthl laccase from T. thermophilus with constitutive promoter J23100, RBS and His-tag

BBa_K863034
BBa_K863034 Version 1 (Component)
pcil35 laccase cDNA sequence from Pycnoporus cinnabarinus

LipA
BBa_K836000 Version 1 (Component)
lipA from B. cepacia (codon usage optimized for E. coli)

DGAT
BBa_K836001 Version 1 (Component)
O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus)

DGAT
BBa_K836002 Version 1 (Component)
O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus) as used

lipA
BBa_K836003 Version 1 (Component)
lipA from B. cepacia (codon usage optimized for E. coli)

BBa_K750005
BBa_K750005 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.01 strength)

BBa_K759003
BBa_K759003 Version 1 (Component)
phaA (beta-ketothiolase orgin from Ralstonia eutropha)

BBa_K759004
BBa_K759004 Version 1 (Component)
phaA (beta-ketothiolase orgin from Ralstonia eutropha)

BBa_K863020
BBa_K863020 Version 1 (Component)
bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag

BBa_K759005
BBa_K759005 Version 1 (Component)
phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)

BBa_K759006
BBa_K759006 Version 1 (Component)
B0034 and phaA (beta-ketothiolase orgin from Ralstonia eutropha)

gpS
BBa_K836004 Version 1 (Component)
Lysis inhibitor from Enterobacteria phage lambda (codon usage optimized for R. opacus)

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

TD1.0
BBa_K750009 Version 1 (Component)
TIME DELAY1.0:LuxI(RBS1.0)->LuxR->LuxPR->GFP

TD0.6
BBa_K750010 Version 1 (Component)
TIME DELAY 0.6:LuxI(RBS0.6)->LuxR->LuxPR->GFP

TD0.3
BBa_K750011 Version 1 (Component)
TIME DELAY0.3:LuxI(RBS0.3)->LuxR->LuxPR->GFP

TD0.01
BBa_K750012 Version 1 (Component)
TIME DELAY0.01:LuxI(RBS0.01)->LuxR->LuxPR->GFP

BBa_K836005
BBa_K836005 Version 1 (Component)
Lysozyme from Rhodococcus phage RER2 (codon usage optimized for R, opacus)

BBa_K759007
BBa_K759007 Version 1 (Component)
RBS(B0034)and phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)

gpS
BBa_K836006 Version 1 (Component)
Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)

nitR
BBa_K836007 Version 1 (Component)
Nitrilase regulator from R. rhodochrous (codon usage optimized for R. opacus)

BBa_K102906
BBa_K102906 Version 1 (Component)
TA8 gate from synthetic algorithm v1.1

BBa_K102907
BBa_K102907 Version 1 (Component)
TA9 gate from synthetic algorithm v1.1

BBa_K102908
BBa_K102908 Version 1 (Component)
TA10 gate from synthetic algorithm v1.1

BBa_K102909
BBa_K102909 Version 1 (Component)
TA11 gate from synthetic algorithm v1.1

BBa_K102910
BBa_K102910 Version 1 (Component)
TA12 gate from synthetic algorithm v1.1

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K131021
BBa_K131021 Version 1 (Component)
R0040+K131016

BBa_K102911
BBa_K102911 Version 1 (Component)
TA13 gate from synthetic algorithm v1.2

p_qrr4
BBa_K131017 Version 1 (Component)
p_qrr4 from <I>Vibrio harveyi</I>

BBa_K131026
BBa_K131026 Version 1 (Component)
AHL inducible GFP

BBa_K102101
BBa_K102101 Version 1 (Component)
TA10 gate in output test harness

BBa_K102111
BBa_K102111 Version 1 (Component)
TA11 gate in output test harness

BBa_K102121
BBa_K102121 Version 1 (Component)
TA12 gate in output test harness

BBa_K102131
BBa_K102131 Version 1 (Component)
TA13 gate in output test harness

BBa_K102141
BBa_K102141 Version 1 (Component)
TA14 gate in output test harness

BBa_K102102
BBa_K102102 Version 1 (Component)
TA10In anti-sense input in test harness

BBa_K102112
BBa_K102112 Version 1 (Component)
TA11In anti-sense input in test harness

BBa_K102122
BBa_K102122 Version 1 (Component)
TA12In anti-sense input in test harness

BBa_K102132
BBa_K102132 Version 1 (Component)
TA13In anti-sense input in test harness

BBa_K102142
BBa_K102142 Version 1 (Component)
TA14In anti-sense input in test harness

BBa_K102905
BBa_K102905 Version 1 (Component)
TA7 gate from synthetic algorithm v1.1

BBa_K082030
BBa_K082030 Version 1 (Component)
luxI generator on PSB3C5

BBa_K082029
BBa_K082029 Version 1 (Component)
LuxI generator