BBa_K292006
BBa_K292006 Version 1 (Component)
Strong RBS + LacI repressor + double terminator

BBa_J05213
BBa_J05213 Version 1 (Component)
Regulator for R3-e2

BBa_K086019
BBa_K086019 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24

BBa_K1758377
BBa_K1758377 Version 1 (Component)
Biosensor device for detection of GHB and GBL

BBa_K1051405
BBa_K1051405 Version 1 (Component)
promoter->ftsQp no RBS

BBa_M50031
BBa_M50031 Version 1 (Component)
pP-T5 promoter

BBa_K336061
BBa_K336061 Version 1 (Component)
promoter+RBS+ompA signal+lacZ+double termnator+promoter+RBS+SBFP2+double termnator

BBa_K119009
BBa_K119009 Version 1 (Component)
cI promoter RcnR op RcnA

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

const.
BBa_R1074 Version 1 (Component)
Constitutive Promoter I

BBa_K780003
BBa_K780003 Version 1 (Component)
Strong constitutive promoter for Bacillus subtilis

BBa_K077004
BBa_K077004 Version 1 (Component)
Hybrid promoter (cI and luxR regulated) and cI lambda

PLac-CI-La
BBa_I763016 Version 1 (Component)
CI and LacY coding regions regulated by PLac Promoter

cI-ptetR
BBa_I1010 Version 1 (Component)
cI(1) fused to tetR promoter

BBa_K1707028
BBa_K1707028 Version 1 (Component)
CFP-ssRA downstream of a cI regulated promoter

BBa_I765007
BBa_I765007 Version 1 (Component)
Fe and UV promoters

BBa_K079040
BBa_K079040 Version 1 (Component)
LexA 2 - opeartor library member

BBa_K1833997
BBa_K1833997 Version 1 (Component)
T3 promoter as annealed oligonucleotides

Pλ
BBa_K1145005 Version 1 (Component)
A promoter which can be inhibited by protein CI

BBa_K091146
BBa_K091146 Version 1 (Component)
pLas/Lux Hybrid Promoter

BBa_K283031
BBa_K283031 Version 1 (Component)
FF_FF ansB promoter (E.coli)

BBa_K1022123
BBa_K1022123 Version 1 (Component)
pTet: cI: TT : pcI: Ulp : Lysis

BBa_K336047
BBa_K336047 Version 1 (Component)
promoter+RBS+SBFP2+double termnator

BBa_K156023
BBa_K156023 Version 1 (Component)
Promoter + RBS + phaC1 + double terminator

BBa_M36136
BBa_M36136 Version 1 (Component)
Rhamnose-inducible promoter

BBa_K2180018
BBa_K2180018 Version 1 (Component)
Promoter PlepA + Operator LacI + Blue Pigment + Double terminator

BBa_K174016
BBa_K174016 Version 1 (Component)
Promoterless ArsR binding site

BBa_R0099
BBa_R0099 Version 1 (Component)
vioA promoter from Chromobacterium Violaceum

BBa_K624029
BBa_K624029 Version 1 (Component)
pYMB+rbs (Pmsp3, 6 bps trunc.) (expression vector essentials on Magnetospirillum magneticum AMB-1)

BBa_K395009
BBa_K395009 Version 1 (Component)
plux(rep)-R0061,small

BBa_M50020
BBa_M50020 Version 1 (Component)
Promoter for GolS + GFP

BBa_K750102
BBa_K750102 Version 1 (Component)
PtetcIT:atc sensor(J23119 promoter) with cI system

BBa_K1088019
BBa_K1088019 Version 1 (Component)
LacI device (constitutive promoter, RBS and terminator)

BBa_K228250
BBa_K228250 Version 1 (Component)
T7 promoter->RBS->cI->Terminator

BBa_K228248
BBa_K228248 Version 1 (Component)
T7 promoter->RBS->cI->Terminator

BBa_K228249
BBa_K228249 Version 1 (Component)
T7 promoter->RBS->cI->Terminator

BBa_K228251
BBa_K228251 Version 1 (Component)
T7 promoter->RBS->cI->Terminator

BBa_K228252
BBa_K228252 Version 1 (Component)
T7 promoter->RBS->cI->Terminator

BBa_K228253
BBa_K228253 Version 1 (Component)
T7 promoter->RBS->cI->Terminator

BBa_K077006
BBa_K077006 Version 1 (Component)
cI (lambda) promoter with LacI

BBa_K228254
BBa_K228254 Version 1 (Component)
T7 promoter->RBS->cI->Terminator

BBa_K228247
BBa_K228247 Version 1 (Component)
T7 promoter->RBS->cI->Terminator

BBa_K1300010
BBa_K1300010 Version 1 (Component)
cI repressible promoter + translational ccdB

BBa_K228246
BBa_K228246 Version 1 (Component)
T7 promoter->RBS->cI->Terminator

BBa_K1026009
BBa_K1026009 Version 1 (Component)
A rationally designed promoter negative regulated by CcaR

BBa_I723011
BBa_I723011 Version 1 (Component)
pDntR (estimated promoter for DntR)

BBa_J85203
BBa_J85203 Version 1 (Component)
J10000.P0440

shTERT
BBa_K1722001 Version 1 (Component)
shTERT is a cancer cell specific promoter with high efficiency.