BBa_I8023
BBa_I8023 Version 1 (Component)
Booperator test: Inverter NOR biasing in polycistronic design

BBa_I8022
BBa_I8022 Version 1 (Component)
Booperator test: Inverter NOR biasing in monocistronic design

BBa_K1994035
BBa_K1994035 Version 1 (Component)
Multiple dCas9 binding site sequence with binding sequence positions shown

BBa_I721004
BBa_I721004 Version 1 (Component)
Lead Binding Protein + Promoter (version a)

BBa_K2082252
BBa_K2082252 Version 1 (Component)
RFP under the control of an optimized lacZ promoter with lambda cI binding site combined with SH2:cI

BBa_J22000
BBa_J22000 Version 1 (Component)
DnaA binding sequence - Px

cln2
BBa_K105014 Version 1 (Component)
cln2 PEST destabilization domain for rapid protein turnover

BBa_K1115006
BBa_K1115006 Version 1 (Component)
dhlB + dhlA ligated with HindIII

BBa_K1461309
BBa_K1461309 Version 1 (Component)
pCMV-RBS-GFP-4 miR 142 5p non-bindgin sites-d.term

BBa_K1777012
BBa_K1777012 Version 1 (Component)
sequence-specific RNA binding protein

BBa_K1777014
BBa_K1777014 Version 1 (Component)
sequence-specific RNA binding protein

BBa_K1777013
BBa_K1777013 Version 1 (Component)
sequence-specific RNA binding protein

BBa_K1777004
BBa_K1777004 Version 1 (Component)
sequence-specific RNA binding protein

BBa_K242300
BBa_K242300 Version 1 (Component)
multi-Ler binding site +promoter 1

BBa_K923005
BBa_K923005 Version 1 (Component)
Ribosome Binding Site in pET14b plasmid

BBa_K1088057
BBa_K1088057 Version 1 (Component)
T25 domain of bacterial two-hybrid system (IPTG inducible)

BBa_K748000
BBa_K748000 Version 1 (Component)
AgrA protein coding sequence. AgrA is the main component of S.aureus agr quorum sensing system.

BBa_K748001
BBa_K748001 Version 1 (Component)
AgrC protein coding sequence. AgrC is main component of S.aureus agr quorum sensing system.

BBa_K1441013
BBa_K1441013 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag INSERT

BBa_J58008
BBa_J58008 Version 1 (Component)
Periplasmic binding protein that docks a vanillin molecule

GFP-AID
BBa_K812110 Version 1 (Component)
GFP fusion with the ubuquitinase E3 OsTirI recoginition domain for PSC2+ plasmid

BBa_K300096
BBa_K300096 Version 1 (Component)
Double phasin and intein separed by a flexible protein domain linker

BBa_J64863
BBa_J64863 Version 1 (Component)
Strong OmpR Binding Operon for Team Challenge 03-2007

BBa_I758600
BBa_I758600 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

BBa_I720010
BBa_I720010 Version 1 (Component)
Ara landing pad (pBBLP 8)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.