Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K112245
BBa_K112245 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!} inserted into K112995 plasmid

BBa_K112246
BBa_K112246 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112995 plasmid

BBa_K112247
BBa_K112247 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!} inserted into K112950 plasmid

BBa_K112248
BBa_K112248 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112950 plasmid

RBS.TetR
BBa_I15040 Version 1 (Component)
RBS.TetR from pASKIBA3

BBa_K112372
BBa_K112372 Version 1 (Component)
{rbs.BgIII}{b1006}

BBa_K112130
BBa_K112130 Version 1 (Component)
Xis.Int with RBS, TrfA with RBS and terminator

BBa_M10092
BBa_M10092 Version 1 (Component)
{Pbad.rbs.prepro˃} For arabinose induced secretion

BBa_M10093
BBa_M10093 Version 1 (Component)
{Pbad.rbs.prepro.StrepTag˃}

BBa_M10212
BBa_M10212 Version 1 (Component)
{Pbad.rbs.prepro.StrepTag}{˂AG4˃}{˂upaG_short!}{dblTerm}

BBa_M10217
BBa_M10217 Version 1 (Component)
{Pbad.rbs.prepro.StrepTag}{˂AG4˃}{˂TshA!}{dblTerm}

BBa_M10213
BBa_M10213 Version 1 (Component)
{Pbad.rbs.prepro.StrepTag}{˂AG4˃}{˂Ag43_short!}{dblTerm}

BBa_M10215
BBa_M10215 Version 1 (Component)
{Pbad.rbs.prepro.StrepTag}{<AG4>}{<ehaB!]{dblTerm}

BBa_M10210
BBa_M10210 Version 1 (Component)
{Pbad.rbs.prepro.StrepTag}{AG4}{CPG_L6!}{dblTerm}

BBa_M10214
BBa_M10214 Version 1 (Component)
{Pbad.rbs.prepro.StrepTag}{&#706;AG4&#707;}{&#706;espP(beta)!}{dblTerm}

BBa_M10216
BBa_M10216 Version 1 (Component)
{Pbad.rbs.prepro.StrepTag}{AG4}{CPompX!}{dblTerm}

BBa_M10225
BBa_M10225 Version 1 (Component)
{Pbad.rbs.prepro.StrepTag}{&#706;GS5-IILK&#707;}{&#706;TshA!}{dblTerm}

BBa_M10211
BBa_M10211 Version 1 (Component)
{Pbad.rbs.prepro.StrepTag}{&#706;AG4&#707;}{&#706;eCPX!}{dblTerm}

BBa_M10221
BBa_M10221 Version 1 (Component)
{Pbad.rbs.prepro.StrepTag}}{&#706;GS5-IILK&#707;}{&#706;Ag43_short!}{dblTerm}

BBa_M10219
BBa_M10219 Version 1 (Component)
{Pbad.rbs.prepro.StrepTag}{&#706;GS5-IILKt&#707;} {&#706;eCPX!}{dblTerm}

BBa_M10223
BBa_M10223 Version 1 (Component)
{Pbad.rbs.prepro.StrepTag}{<GS5-IILK>}{<ehaB!]{dblTerm}

BBa_M10220
BBa_M10220 Version 1 (Component)
{Pbad.rbs.prepro.StrepTag}{&#706;GS5-IILK&#707;}{&#706;upaG_short!}{dblTerm}

BBa_M10218
BBa_M10218 Version 1 (Component)
{Pbad.rbs.prepro.StrepTag}{GS5-IILK}{CPG_L6!}{dblTerm}

BBa_M10224
BBa_M10224 Version 1 (Component)
{Pbad.rbs.prepro.StrepTag}{GS5-IILK}{CPompX!}{dblTerm}

BBa_M10222
BBa_M10222 Version 1 (Component)
{Pbad.rbs.prepro.StrepTag}{&#706;GS5-IILK&#707;}{&#706;espP(beta)!}{dblTerm}

BBa_P101101
BBa_P101101 Version 1 (Component)
SAMT RBS.ORF.Term

BBa_P101102
BBa_P101102 Version 1 (Component)
BAMT RBS.ORF.Term

BBa_P101103
BBa_P101103 Version 1 (Component)
JMT RBS.ORF.Term (PCR)

BBa_P101104
BBa_P101104 Version 1 (Component)
JMT RBS.ORF.Term (Synth)

BBa_P101105
BBa_P101105 Version 1 (Component)
SAAT RBS.ORF.Term

BBa_P101106
BBa_P101106 Version 1 (Component)
BEAT RBS.ORF.Term

BBa_P101107
BBa_P101107 Version 1 (Component)
EHT1 RBS.ORF.Term

BBa_P101108
BBa_P101108 Version 1 (Component)
EEB1 RBS.ORF.Term

BBa_P101109
BBa_P101109 Version 1 (Component)
LMS RBS.ORF.Term

BBa_P101110
BBa_P101110 Version 1 (Component)
LIS RBS.ORF.Term

BBa_P101111
BBa_P101111 Version 1 (Component)
Oe23 RBS.ORF.Term

BBa_P101112
BBa_P101112 Version 1 (Component)
Myrcene Synthase RBS.ORF.Term

BBa_P101113
BBa_P101113 Version 1 (Component)
PAD1 RBS.ORF.Term

BBa_K200005
BBa_K200005 Version 1 (Component)
OtsA: Part 1 of 2 for trehalose producing enzymes.

tADH1
BBa_J63002 Version 1 (Component)
ADH1 terminator from S. cerevisiae

BBa_K177033
BBa_K177033 Version 1 (Component)
pcI lam RBS.3 LacI pcl lam RBS mRFP1 terminator

BBa_K177035
BBa_K177035 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA) under control of RBS.3 (medium)

BBa_K177036
BBa_K177036 Version 1 (Component)
CFP protein under RBS.3 (medium)

BBa_K177041
BBa_K177041 Version 1 (Component)
p53 CDS under RBS.3 (medium)

BBa_K177042
BBa_K177042 Version 1 (Component)
Yellow Fluorescent Protein under RBS.3 (medium)

BBa_K177043
BBa_K177043 Version 1 (Component)
Tetracycline repressor under RBS.3 (medium)

BBa_K177044
BBa_K177044 Version 1 (Component)
CI repressor from E. coli phage lambda + Tetracycline repressor (both under RBS.3)

adh2
BBa_K273005 Version 1 (Component)
Alcohol Dehydrogenase 2 (adh2 , adhII, adhB) from <i>S. cerevisiae</i>

BBa_K273007
BBa_K273007 Version 1 (Component)
RBS - Alcohol Dehydrogenase 2 (adh2 , adhII, adhB) from <i>S. cerevisiae</i>