BBa_K2123111
BBa_K2123111 Version 1 (Component)
Tac promoter with downstream mer operator + RFP (K081014)

BBa_K1795400
BBa_K1795400 Version 1 (Component)
Truncated pSB1C3 with no Prefix or Suffix

BBa_K1151030
BBa_K1151030 Version 1 (Component)
pint-RBS-GFP-TER-pUreA-RBS-RFP (LVAtag)

BBa_K1151028
BBa_K1151028 Version 1 (Component)
pexbB-RBS-GFP-TER-pUreA-RBS-RFP (LVAtag)

BBa_K1151029
BBa_K1151029 Version 1 (Component)
pnikR-RBS-GFP-TER-pUreA-RBS-RFP (LVAtag)

BBa_K228013
BBa_K228013 Version 1 (Component)
(pSal PO) OR Gate - GFP

BBa_K1363401
BBa_K1363401 Version 1 (Component)
the downstream passway of the red color system

BBa_S03638
BBa_S03638 Version 1 (Component)
HixC-pBad_rev-HixC : RBS-TetF

BBa_M11085
BBa_M11085 Version 1 (Component)
E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o

BBa_K101013
BBa_K101013 Version 1 (Component)
Construct with TetR/p22cII promoter, RBS, RFP, Terminator

BBa_K2123113
BBa_K2123113 Version 1 (Component)
Stationary phase promoter with downstream mer operator + RFP (K081014)

BBa_J162001
BBa_J162001 Version 1 (Component)
RBS cl RBS RFP RBS 3OC6HSL Double Terminator

BBa_K101012
BBa_K101012 Version 1 (Component)
Construct with TetR/p22cII promoter, RBS, RFP, Terminator

BBa_K411123
BBa_K411123 Version 1 (Component)
Split RFP-eIF4A_A Generator (R0040 + B0034 + RFP_split_A+Linker+eIF4A_split_A + B0015)

BBa_K415011
BBa_K415011 Version 1 (Component)
PtetR : RBS : LuxR : Term : PluxR/cI-OR : RBS : mCherry : Term : Plux/cI-OR : RBS : LuxI

SET
BBa_K1433011 Version 1 (Component)
Terminator-RFP-RBS-attB-P-attP-RBS-GFP-Terminator

BBa_S03737
BBa_S03737 Version 1 (Component)
pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB1A2)

BBa_S03674
BBa_S03674 Version 1 (Component)
(-1,2) HixC-pBad_rev-HixC : RBS-TetF-HixC

BBa_K092500
BBa_K092500 Version 1 (Component)
TetR cds with RBS and Terminator + pTetR, RFP with RBS

BBa_K2123112
BBa_K2123112 Version 1 (Component)
Tac promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)

BBa_K1067000
BBa_K1067000 Version 1 (Component)
Periplasmic directed GFP SF with signal peptide TAT and RFP as background color

BBa_J22121
BBa_J22121 Version 1 (Component)
Lac Y gene under the rec A(SOS) promoter in plasmid pSB2K3

BBa_K2123114
BBa_K2123114 Version 1 (Component)
Stationary phase promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)

GG100
BBa_K2145125 Version 1 (Component)
This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer

GG98
BBa_K2145123 Version 1 (Component)
This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer

BBa_K2123117
BBa_K2123117 Version 1 (Component)
Novel RFP device regulated by mercury: MerR (regulatory protein) + Stationary phase with mer operato

BBa_K2123116
BBa_K2123116 Version 1 (Component)
Universal promoter for both phase of growth in tandem with downstram mer operator + RFP (K081014)

BBa_K415005
BBa_K415005 Version 1 (Component)
pLux/cI-OR : RBS-mCherry : Term : p(tetR) : RBS-luxR : Term

BBa_K1520509
BBa_K1520509 Version 1 (Component)
PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.