BBa_K1824890
BBa_K1824890 Version 1 (Component)
The Sequence of the SpeI/XbaI scar in BioBricks standard assembly.

BBa_K1159309
BBa_K1159309 Version 1 (Component)
19 AA Linker with N-terminal <i>Strep</i>-tag II and C-terminal TEV cleavage site in RFC[25]

BBa_K1945002
BBa_K1945002 Version 1 (Component)
Homologous Recombination Intermediate for Counter-selection in Synechocystis sp. PCC 6803

BBa_K322128
BBa_K322128 Version 1 (Component)
Phycocyanobilin synthesis genes + Red light detection system (EYFP as reporter)

BBa_K1893034
BBa_K1893034 Version 1 (Component)
Scar

BBa_M1680
BBa_M1680 Version 1 (Component)
Standard parts assembly for detection of high glucose levels using FAS promoter

BBa_K782049
BBa_K782049 Version 1 (Component)
HindIII/SacI equivalent multicloning site for insertion of non standardized parts to BioBrick vector

BBa_K782052
BBa_K782052 Version 1 (Component)
BamHI/SacI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782058
BBa_K782058 Version 1 (Component)
SacI/XhoI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782057
BBa_K782057 Version 1 (Component)
SacI/SpeI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782055
BBa_K782055 Version 1 (Component)
XbaI/SacI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782045
BBa_K782045 Version 1 (Component)
EcoRI/SacI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782056
BBa_K782056 Version 1 (Component)
SacI/BamHI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K887003
BBa_K887003 Version 1 (Component)
Plac+B0034+alsS+zif268+B0034+PBS II+ilvC+B0034+HIVC+ilvD+Ptet+B0032+kivD+B0015

BBa_K1716002
BBa_K1716002 Version 1 (Component)
NahR Biosensor for detection of acetylsalicylic acid/aspirin with blue chromoprotein reporter

luxR-ndh
BBa_K1036000 Version 1 (Component)
lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II

BBa_M45905
BBa_M45905 Version 1 (Component)
Composite part for fluorescent detection of botulinum neurotoxin A (BoNT A)

BBa_M31513
BBa_M31513 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III

BBa_M31516
BBa_M31516 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III

BBa_K299801
BBa_K299801 Version 1 (Component)
standard biobrick scar (if next sequence does NOT start with ATG)

BBa_K1833996
BBa_K1833996 Version 1 (Component)
Oligonucleotide synthesis scar

BBa_K1897038
BBa_K1897038 Version 1 (Component)
Scar site generated by 3A Assembly

Strep-Tag
BBa_I757014 Version 1 (Component)
Strep-Tag II affinity tag

BBa_K1583054
BBa_K1583054 Version 1 (Component)
Non-coding scar site in between RBS and gene

BBa_K2055011
BBa_K2055011 Version 1 (Component)
8 bp Scar

BBa_K2055008
BBa_K2055008 Version 1 (Component)
6 bp Scar

BBa_K1366101
BBa_K1366101 Version 1 (Component)
Genetic construct for lpp gene deletion (C1)

BBa_K1897039
BBa_K1897039 Version 1 (Component)
Scar site generated by 3A Assembly

BBa_K1159991
BBa_K1159991 Version 1 (Component)
10/10 NC Assembly Scar

BBa_M45102
BBa_M45102 Version 1 (Component)
Cobalt detection Biobrick. RFP made in presence of Cobalt. Note the receptor also works in the prese

BBa_K299800
BBa_K299800 Version 1 (Component)
standard biobrick scar (if next part starts with ATG)

BBa_K1159992
BBa_K1159992 Version 1 (Component)
10/10 C Assembly Scar

BBa_K1452000
BBa_K1452000 Version 1 (Component)
Pos/neg selection (Bacillus)

BBa_K142205
BBa_K142205 Version 1 (Component)
Plac + RBS + SceI + double terminator

BBa_M31114
BBa_M31114 Version 1 (Component)
M13, gene II from HpaI to the end [1-831bp]

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.