M13 -47
BBa_K176059 Version 1 (Component)
M13 -47 general primer as a reverse primer binds to 5prime terminal of lacZ

pLacIQ1-RB
BBa_K193406 Version 1 (Component)
the coding is the same as BBa_193404 the back bone is changed onto pSB6A1

BBa_K1072007
BBa_K1072007 Version 1 (Component)
Lux pI+RBS+LuxR+2TM+Lux pR+RBS+LuxI+2TM+Lux pR+RBS+GFP+2TM+Lux pR+RBS+α-ALS+2TM

BBa_K1231005
BBa_K1231005 Version 1 (Component)
Asr-128-Lpp-RBS-GFP

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K1412089
BBa_K1412089 Version 1 (Component)
Riboregulator which combines crRNA and RBS acting as a lock to the gene cuicuit

BBa_J100198
BBa_J100198 Version 1 (Component)
J100198 contains the fitness gene tester construct (J100199) with thymidylate synthase as the fitnes

DGAT
BBa_K836002 Version 1 (Component)
O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus) as used

BBa_K1230003
BBa_K1230003 Version 1 (Component)
RBS-GFP-Terminator with LL-37 antimicrobial peptide with modified codon usage for E. coli as prefix

BBa_J70652
BBa_J70652 Version 1 (Component)
This tests adding an internal strep tag linker to a protein using J70590 as a te

BBa_K563004
BBa_K563004 Version 1 (Component)
pTEF1, promoter of Translational elongation factor EF-1 alpha

BBa_K1447002
BBa_K1447002 Version 1 (Component)
Epitope 2 from Ara h 1

BBa_J70653
BBa_J70653 Version 1 (Component)
This tests adding an internal strep tag linker to a protein using J70590 as a te

BBa_K2097000
BBa_K2097000 Version 1 (Component)
CpxR binding site attached to a yellow-green color protein (YGCP) acts as a neutral pH indicator.

BBa_K2092004
BBa_K2092004 Version 1 (Component)
alcR (incl RBS), ethanol-activated transcription factor from A. nidulans

BBa_K1362061
BBa_K1362061 Version 1 (Component)
N-terminal chitin binding domain with triglycine linker and His-tag as RFC[105] insert

BBa_K1447004
BBa_K1447004 Version 1 (Component)
Epitope 1-5 from Ara h 1

BBa_K1447005
BBa_K1447005 Version 1 (Component)
Epitope 1-5 from Ara h 1

iGEM Parts Registry
igem_collection Version 1 (Collection)
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.

BBa_K1778005
BBa_K1778005 Version 1 (Component)
eGFP:enhanced Green Fluorescent Protein. It???s the mutant of GFP. It is widely used as report gene

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

CMV + GFP
BBa_K1852000 Version 1 (Component)
GFP + CMV Promoter used for expression in oocyte cells. This part was planned to be used as a report

BBa_K1231003
BBa_K1231003 Version 1 (Component)
Asr-64-Lpp-RBS-GFP is a dual-state construct

BBa_K603001
BBa_K603001 Version 1 (Component)
mitochondrial proteorhodopsin WZK (same as K603000)

BBa_K1412088
BBa_K1412088 Version 1 (Component)
A combination of theophylline aptamer and taRNA that can response theophylline to regulate circuit

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

pCMV-ECFP-
BBa_I763023 Version 1 (Component)
LacI coding device with ECFP as a reporter regulated by pCMV

BBa_J24823
BBa_J24823 Version 1 (Component)
same as J24819 but with ACCACC Euk RBS removed and problem solved via J24822 removed

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.