BBa_K1891010
BBa_K1891010 Version 1 (Component)
YCE+alpha-tubulin

BBa_K782060
BBa_K782060 Version 1 (Component)
Interferon alpha-2a

402
BBa_K398402 Version 1 (Component)
Prefoldin-alpha generator

BBa_K1891009
BBa_K1891009 Version 1 (Component)
YNE+alpha-tubulin

BBa_J17003
BBa_J17003 Version 1 (Component)
G-alpha-olf

BBa_K1891007
BBa_K1891007 Version 1 (Component)
alpha-tubulin+YNE

BBa_K1891008
BBa_K1891008 Version 1 (Component)
alpha-tubulin+YCE

BBa_K539742
BBa_K539742 Version 1 (Component)
TetR repressible promoter (BBa_R0040)+ kivd (alpha-ketoisovalerate decarboxylase)+Ter (BBa_B0015)

BBa_K234092
BBa_K234092 Version 1 (Component)
Stop codon + CYC1 Terminator

BBa_K611049
BBa_K611049 Version 1 (Component)
pTet Variant #9

GeA
BBa_K411101 Version 1 (Component)
GFP_split_A + Linker + eIF4A_split_A

BBa_K2036028
BBa_K2036028 Version 1 (Component)
placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-ompA-iLDH-TT-pRM-RBS-beta-galactosidase

BBa_K861045
BBa_K861045 Version 1 (Component)
S-FadA with a RBS and a terminator

BBa_K265011
BBa_K265011 Version 1 (Component)
OmpA with proteolytic cleavage tag

BBa_K252007
BBa_K252007 Version 1 (Component)
OmpA target sequence for MicA

BBa_K156033
BBa_K156033 Version 1 (Component)
RBS + phaB1 + double terminator

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K2185006
BBa_K2185006 Version 1 (Component)
S.ferax alpha amylase coding sequence

pTet-amilC
BBa_K1455001 Version 1 (Component)
pTet - amilCP

pTet-petF
BBa_K2135003 Version 1 (Component)
petF on pTet inducible promoter

BBa_K2041011
BBa_K2041011 Version 1 (Component)
ptet-RBS-tetR-fimE-T-pbad-RBS-Bxb1-T

BBa_K1314004
BBa_K1314004 Version 1 (Component)
Ptet mRFP with random sequence

BBa_K199043
BBa_K199043 Version 1 (Component)
pOmpC-RBS-Tet

BBa_S03715
BBa_S03715 Version 1 (Component)
lox-RBS-Tet

BBa_K199044
BBa_K199044 Version 1 (Component)
pLux-RBS-Tet

BBa_K678039
BBa_K678039 Version 1 (Component)
T3-motni, terminator for Aspergillus niger

BBa_K678038
BBa_K678038 Version 1 (Component)
T2-motni, terminator for Aspergillus niger

BBa_Z0271
BBa_Z0271 Version 1 (Component)
T7.2 TE transcriptional terminator

BBa_K199176
BBa_K199176 Version 1 (Component)
pT7+RBS+GFP

ReB
BBa_K411104 Version 1 (Component)
RFP_split_B + Linker + eIF4A_split_B

BBa_K1520510
BBa_K1520510 Version 1 (Component)
PgolTS-golS-PgolB-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter

BBa_M1021
BBa_M1021 Version 1 (Component)
RBS+ "Blues" Zinc Finger + PhaC

BBa_K1994045
BBa_K1994045 Version 1 (Component)
T7 terminator SBa_000587

BBa_K199157
BBa_K199157 Version 1 (Component)
pLac-RBS-CCACU-RFP-pLpp-CUAGU tRNA-pLpp-CCACU tRNA

BBa_K533002
BBa_K533002 Version 1 (Component)
OmpA-HIV protease site-SH3

BBa_K827015
BBa_K827015 Version 1 (Component)
artificial designed terminator

BBa_M31905
BBa_M31905 Version 1 (Component)
Gene 5 RBS, Promoter, and ORF

BBa_K654042
BBa_K654042 Version 1 (Component)
E. coli 1.0 RBS + Firefly Luciferase + Terminator

BBa_K1230012
BBa_K1230012 Version 1 (Component)
rbs-GFP-terminator-pLsrA-LL37

sam8 + rbs
BBa_K238027 Version 1 (Component)
Sam8 + RBS

tetR
BBa_Q01400 Version 1 (Component)
QPI (TetR, Medium RBS)

BBa_K199094
BBa_K199094 Version 1 (Component)
pTet-CCACU tRNA-pLac-RBS-CCACU-RFP

BBa_K199095
BBa_K199095 Version 1 (Component)
pLac-RBS-CCACU-RFP-pTet-CCACU tRNA

BBa_K199092
BBa_K199092 Version 1 (Component)
pTet-CGGUC tRNA-pLac-RBS-CGGUC-RFP

BBa_K199093
BBa_K199093 Version 1 (Component)
pLac-RBS-CGGUC-RFP-pTet-CGGUC tRNA