pSB1K15
pSB1K15 Version 1 (Component)
Gateway entry vector for promoters (L4R1)

BBa_K590064
BBa_K590064 Version 1 (Component)
The FabBrick: FabH2, an enzyme for changing up fatty acid biosynthesis

pSB1K16
pSB1K16 Version 1 (Component)
Gateway entry vector for gene cassette (L1L2)

Co-op
BBa_K233002 Version 1 (Component)
Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway

BBa_K233001
BBa_K233001 Version 1 (Component)
Snowdrift 1 , using TorA. A module to secrete B-galactosidase using the TAT-export Pathway

BBa_K1363401
BBa_K1363401 Version 1 (Component)
the downstream passway of the red color system

BBa_K563010
BBa_K563010 Version 1 (Component)
Tor2 gene from the genome of the S. cerevisiae, central protein in TOR(target of rapamycin) pathway

BBa_K608001
BBa_K608001 Version 1 (Component)
First of two requisite genes required for the biosynthesis of phycocyanobilin from heme combined wit

BBa_K2044001
BBa_K2044001 Version 1 (Component)
Based on our project, <2-4-8> is a feasible pathway from Site No. 2 to Site No. 8

BBa_K2044000
BBa_K2044000 Version 1 (Component)
Based on our project, <2-6-8> is the optimal pathway scheme from Site No. 2 to Site No. 8

BBa_K2044010
BBa_K2044010 Version 1 (Component)
Based on our project, <5,7> is the direct pathway from Site No.5to Site No.7 in the map we design.

BBa_K2044006
BBa_K2044006 Version 1 (Component)
Based on our project,<3,6> is the direct pathway from Site No.3 to Site No.6 in the map we design.

BBa_K2044004
BBa_K2044004 Version 1 (Component)
Based on our project, <2,4> is the direct pathway from Site No.2 to Site No.4 in the map we design.

BBa_K2044009
BBa_K2044009 Version 1 (Component)
Based on our project, <4,8> is the direct pathway from Site No.4 to Site No.8 in the map we design.

BBa_K2044014
BBa_K2044014 Version 1 (Component)
Based on our project, <1,4> is the direct pathway from Site No.1 to Site No.4 in the map we design.

BBa_K2044007
BBa_K2044007 Version 1 (Component)
Based on our project, <4,5> is the direct pathway from Site No.4 to Site No.5 in the map we design.

BBa_K2044013
BBa_K2044013 Version 1 (Component)
Based on our project,<7,8> is the direct pathway from Site No.7 to Site No.8 in the map we design.

BBa_K2044008
BBa_K2044008 Version 1 (Component)
Based on our project, <4,6> is the direct pathway from Site No.4 to Site No.6 in the map we design.

BBa_K2044003
BBa_K2044003 Version 1 (Component)
Based on our project, <2,3> is the direct pathway from Site No.2 to Site No.3 in the map we design.

BBa_K2044002
BBa_K2044002 Version 1 (Component)
Based on our project, <2,1> is the direct pathway from Site No.2 to Site No.1 in the map we design.

BBa_K2044005
BBa_K2044005 Version 1 (Component)
Based on our project, <2,6> is the direct pathway from Site No.2 to Site No.6 in the map we design.

BBa_K2044012
BBa_K2044012 Version 1 (Component)
Based on our project, <6,8> is the direct pathway from Site No.6 to Site No.8 in the map we design.

BBa_K2044011
BBa_K2044011 Version 1 (Component)
Based on our project,<6,4> is the direct pathway from Site No.6 to Site No.4 in the map we design.

BBa_K1363005
BBa_K1363005 Version 1 (Component)
R0082-HfiA the HfiA protein express passway which can be regulated by light

BBa_S04147
BBa_S04147 Version 1 (Component)
The Partial Butanol Operon (5 inserts from I725021 to I725025)

BBa_I754000
BBa_I754000 Version 1 (Component)
Feruloyl CoA Hyratase for vanillin biosynthesis-ech

BBa_I730000
BBa_I730000 Version 1 (Component)
Feruloyl CoA hydratase for vanillin biosynthesis

BBa_K527001
BBa_K527001 Version 1 (Component)
Biosynthesis/Oximes/CYP79A2/Plug 'n' Play

BBa_I738002
BBa_I738002 Version 1 (Component)
feruloyl-CoA hydratase enzyme for biosynthesis of vanillin

BBa_K1051262
BBa_K1051262 Version 1 (Component)
The measurement pathway of degradation tag K1051208.

BBa_K1051260
BBa_K1051260 Version 1 (Component)
The measurement pathway of degradation tag K1051206.

BBa_K1051261
BBa_K1051261 Version 1 (Component)
The measurement pathway of degradation tag K1051207.

BBa_I757001
BBa_I757001 Version 1 (Component)
Feruloy CoA hydratase 4 vanillin biosynthesis

BBa_I754001
BBa_I754001 Version 1 (Component)
ech protein generator for vanillin biosynthesis

BBa_I769106
BBa_I769106 Version 1 (Component)
ech protein generator for vanillin biosynthesis

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.