BBa_K1444024BBa_K1444024 Version 1 (Component)Composite promoter and weak B.subtilis RBS - C1-434 x3
BBa_I766346BBa_I766346 Version 1 (Component)ING2 PH - mCherry under medium constitutive promoter
BBa_K176248BBa_K176248 Version 1 (Component)Low input score automatic selector (with reporter): PoPS->mRFP->CI Inverter (cI-LVA+pCI)->K176229
BBa_K1444002BBa_K1444002 Version 1 (Component)Composite promoter and consensus <i>B.subtilis</i> RBS - <i>C1-λ</i>
BBa_K116001BBa_K116001 Version 1 (Component)nhaA promoter, that can be regulated by pH and nhaR protein.
BBa_K1510020BBa_K1510020 Version 1 (Component)It is a promoter which is induced by low pH.
BBa_K648027BBa_K648027 Version 1 (Component)c1 repressor with a very weak RBS on a promoter
BBa_I766235BBa_I766235 Version 1 (Component)Ent5p-ENTH PH -GFP under medium constitutive promoter
BBa_K1962013BBa_K1962013 Version 1 (Component)A pH Sensing Device Based on The GadA Promoter
BBa_K1962014BBa_K1962014 Version 1 (Component)A pH Sensing Device Based on The Asr Promoter
BBa_K1111003BBa_K1111003 Version 1 (Component)Cad Promoter induced by low pH and high lysine conentrations
BBa_K176249BBa_K176249 Version 1 (Component)Low input score automatic selector w/o lethal device(with reporter): PoPS->mRFP->CI-LVA+pCI->K176240
phrI-P1BO_2870 Version 1 (Component) BBa_K609000BBa_K609000 Version 1 (Component)nisI+pH induced promoter rcfB+ repressor LacR of lac operon
BBa_K1444004BBa_K1444004 Version 1 (Component)Composite promoter and weak <i>B. subtilis </i> RBS - <i>C1-λ</i>
BBa_K1962016BBa_K1962016 Version 1 (Component)A device for expression of Im-E3 in response to pH
BBa_K1962015BBa_K1962015 Version 1 (Component)A device for expression of Im-Ia in response to pH
BBa_K817003BBa_K817003 Version 1 (Component)Novel thermal promoter, Phs
BBa_K1022129BBa_K1022129 Version 1 (Component)pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Magainin II:RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Ly
BBa_K1022128BBa_K1022128 Version 1 (Component)pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Signiferin: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Ly
BBa_K1022127BBa_K1022127 Version 1 (Component)pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: MaximinH5: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Lys
BBa_I751201BBa_I751201 Version 1 (Component)pcI-luxI on pSB4
BBa_K1124108BBa_K1124108 Version 1 (Component)pLambda-mCherry-pConst-c1 repressor generator (-LVA, -barcode, E234K) (for UV induction check)
BBa_K1444003BBa_K1444003 Version 1 (Component)Composite promoter and consensus <i>B. subtilis</i> RBS - <i>C1-λ</i> x3
BBa_K318521BBa_K318521 Version 1 (Component)Encryption Lysis - pLock Lysis p(pH) Hin pBile Cre pCons ramA
BBa_K896599BBa_K896599 Version 1 (Component)Strophurus rankini voucher AMS R140490 NADH dehydrogenase subunit 4 (ND4) gene, partial cds; tRNA-Hi
BBa_K2097000BBa_K2097000 Version 1 (Component)CpxR binding site attached to a yellow-green color protein (YGCP) acts as a neutral pH indicator.
IBMc378IBMc378 Version 1 (Component)pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-17)-PI-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(18-154)-S-mCherry*(193-236)-H6-T
BBa_K1231001BBa_K1231001 Version 1 (Component)GadA-RBS is a pH sensitive promoter
BBa_K1072007BBa_K1072007 Version 1 (Component)Lux pI+RBS+LuxR+2TM+Lux pR+RBS+LuxI+2TM+Lux pR+RBS+GFP+2TM+Lux pR+RBS+α-ALS+2TM
BBa_K1072022BBa_K1072022 Version 1 (Component)Lux pI+RBS+LuxR+2TM+Lux pR+RBS+LuxI+2TM+Lux pR+RBS+GFP+2TM+Lux pR+RBS+Ndh+2TM
BBa_K1444010BBa_K1444010 Version 1 (Component)Composite promoter and weak B. subtilis RBS - C1-434
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.