BBa_K1361004BBa_K1361004 Version 1 (Component)Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag
BBa_K2082237BBa_K2082237 Version 1 (Component)RFP and Tetracycline resistence(tetA) under the control of a modified lacZ promoter; Fusion protein
BBa_K1994060BBa_K1994060 Version 1 (Component)Multiple dCas9 binding sites with weak sigma 54 promoter upstream of GFP + dCas9 expression cassette
BBa_K079049BBa_K079049 Version 1 (Component)GFP reporter protein under the control of the J23118 constitutive promoter and LexA 2 operator
Altered Sequence ActivitiesAlteredSequenceActivities_collection Version 1 (Collection)A set of activities to point to ways in which sequences have been altered between source and upload
BBa_K079020BBa_K079020 Version 1 (Component)GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein
BBa_K1109007BBa_K1109007 Version 1 (Component)FepA L8T Mutant -composite of Large Diffusion pore for E. coli outer membrane coding sequence transc
BBa_J85010BBa_J85010 Version 1 (Component)PLux/cI-OR Promoter
BBa_K1778005BBa_K1778005 Version 1 (Component)eGFP:enhanced Green Fluorescent Protein. It???s the mutant of GFP. It is widely used as report gene
BBa_M11410BBa_M11410 Version 1 (Component)Type 2 promoter of sigE gene. Sigma factor regulates light and nitrogen responses, and has been obse
BBa_J31016BBa_J31016 Version 1 (Component)part produces the RNA construct crRNA-RBS-GFPLVA-tt that can only be translated in the presence of t
BBa_K2088006BBa_K2088006 Version 1 (Component)It encodes a kind of protein named 2Fe-2S ferredoxin, a 2Fe-2S iron-sulfur cluster binding domain. I
Bm3R1BBa_K1401000 Version 1 (Component)TetR homolog. This is a MoClo part of the Bm3R1 repressor gene with CD fusion sites ('AATG', 'AGGT')
BBa_I733007BBa_I733007 Version 1 (Component)Weight cells either turn blue or die in response to both inputs and HSL level
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_K1189029BBa_K1189029 Version 1 (Component)TALE-A with a his tag linked to a K coil under the control of a LacI promoter
BBa_M45102BBa_M45102 Version 1 (Component)Cobalt detection Biobrick. RFP made in presence of Cobalt. Note the receptor also works in the prese
BBa_M31513BBa_M31513 Version 1 (Component)Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III
BBa_M31516BBa_M31516 Version 1 (Component)Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III
YFP_SPferBBa_K809314 Version 1 (Component)YFP + signal peptide of FER
iGEM Parts Registryigem_collection Version 1 (Collection)The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.
BBa_K987000BBa_K987000 Version 1 (Component)This part is a coding part that produces Vip3Ca3, a protein that can deals with different forms of p
BBa_I13035BBa_I13035 Version 1 (Component)3OC<sub>6</sub>HSL Receiver Device with Inducible Control of LuxR and a YFP Output device
BBa_I741109BBa_I741109 Version 1 (Component)Lambda Or operator region
BBa_K189060BBa_K189060 Version 1 (Component)Gp15 of Bacteriophage Mu
BBa_M11085BBa_M11085 Version 1 (Component)E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o
BBa_K1676120BBa_K1676120 Version 1 (Component)Mutant 16 of Lactate Dehydrogenase
BBa_K1676114BBa_K1676114 Version 1 (Component)Mutant 10 of Lactate Dehydrogenase
BBa_K1361007BBa_K1361007 Version 1 (Component)Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative
BBa_K2082252BBa_K2082252 Version 1 (Component)RFP under the control of an optimized lacZ promoter with lambda cI binding site combined with SH2:cI
BBa_K1228001BBa_K1228001 Version 1 (Component)A fragment of loctoferrin
BBa_K2041010BBa_K2041010 Version 1 (Component)plac-RBS-Bxb1-T-plux-RBS-luxR-T-plux-recognition site of Bxb1-RBS-luxI-RBS-GFP-T
BBa_K809108BBa_K809108 Version 1 (Component)Efficiency test of Q0255 Terminator
RFP_SPtim2BBa_K809303 Version 1 (Component)RFP + signal peptide of TIM21
BBa_K337088BBa_K337088 Version 1 (Component)Fragment 7 of wt AAV6
BBa_K337060BBa_K337060 Version 1 (Component)Fragment 3 of wt AAV1
T25 domainBBa_K1088056 Version 1 (Component)T25 domain of CyaA from Bordetella pertussis
BBa_K1795400BBa_K1795400 Version 1 (Component)Truncated pSB1C3 with no Prefix or Suffix
BBa_K1351017BBa_K1351017 Version 1 (Component)SdpI with RBS: Immunity against the cannibalsim toxin sdpC of <i>B. subtilis</i>
BBa_M31786BBa_M31786 Version 1 (Component)1st half of Gene III- preBamHI cut