BBa_K1747000
BBa_K1747000 Version 1 (Component)
Diagnostic circuit using GFP to evaluate fatty acid promoter repsonse

BBa_J85006
BBa_J85006 Version 1 (Component)
3OC6HSL -> non-functional RBS, RFP, terminator; constitutive GFP

BBa_K1128002
BBa_K1128002 Version 1 (Component)
M.SssI with a linker - ready for fusing

BBa_J70084
BBa_J70084 Version 1 (Component)
Adds 6 his suffix, using BioScaffold part J70030 (PpiI) in pSB1AT3

Pveg Xyle
BBa_K316005 Version 1 (Component)
Functional XylE under Pveg promoter, with double terminator

BBa_K316004
BBa_K316004 Version 1 (Component)
Functional XylE under J23101 promoter, with double terminator

BBa_M1680
BBa_M1680 Version 1 (Component)
Standard parts assembly for detection of high glucose levels using FAS promoter

BBa_J119315
BBa_J119315 Version 1 (Component)
Scaffold 2.0 for J-GGA (With the promoter between Junction A and B)

BBa_J24822
BBa_J24822 Version 1 (Component)
Same as J24819 but with the error at the luc-terminator junction fixed

BBa_J119142
BBa_J119142 Version 1 (Component)
Part for inserting modified D-Dogs for Golden Gate Assembly using BsgI

BY-Toggle
BBa_K1908000 Version 1 (Component)
Blue-Yellow fluorescent protein toggle switch using pLac and OmpC promoter.

BBa_J119300
BBa_J119300 Version 1 (Component)
Part for inserting modified D-Dogs for Golden Gate Assembly using BsgI

PrtDEF
BBa_K258008 Version 1 (Component)
Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi

BBa_M50053
BBa_M50053 Version 1 (Component)
FRET-based glucose sensor using a glucose binding protein, mRuby3 and cometGFP

TSensor
BBa_J11021 Version 1 (Component)
Tunable Temperature Sensor v2 (using LacI ts + other parts from Registry 7.05)

BBa_K987001
BBa_K987001 Version 1 (Component)
This is a composite part which has the function to invert the temperature activation by the part: BB

BBa_J70652
BBa_J70652 Version 1 (Component)
This tests adding an internal strep tag linker to a protein using J70590 as a te

BBa_J70653
BBa_J70653 Version 1 (Component)
This tests adding an internal strep tag linker to a protein using J70590 as a te

BBa_K1648004
BBa_K1648004 Version 1 (Component)
Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane

BBa_K1465223
BBa_K1465223 Version 1 (Component)
Functional carboxysome of Halothiobacillus neapolitanus with a GFP fusion under T7 control

BBa_K737000
BBa_K737000 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K737001
BBa_K737001 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K774102
BBa_K774102 Version 1 (Component)
Multi sensor - for calculation of specific concentrations of nitrates nitrites and nitric oxide usin

PrtDEF
BBa_K258007 Version 1 (Component)
Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi

Bacillus subtilis Collection
bsu_collection Version 1 (Collection)
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.