xis domain
BBa_K112203 Version 1 (Component)
{< xis >} The bacteriophage lambda xis gene without start or stop codons; assembly standard 21

BBa_J70559
BBa_J70559 Version 1 (Component)
N-terminal fusion test: R0010:J70558 or R0010:J70550:E0040

BBa_K363002
BBa_K363002 Version 1 (Component)
A calcium dependent response element binding site for the Crz1 activator

BBa_I758600
BBa_I758600 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

BBa_K563053
BBa_K563053 Version 1 (Component)
vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.

BBa_K1440002
BBa_K1440002 Version 1 (Component)
part1 or our whole design,SV40>PTRE>EYFP>loxP>Ribozyme>SV40

BBa_K1942000
BBa_K1942000 Version 1 (Component)
A shRNA corresponding DNA sequence for KRAS which could silence the gene

BBa_K2016006
BBa_K2016006 Version 1 (Component)
Superfolder GFP under control or medium constitutive promoter and RyhB siRNA domain

BBa_K415001
BBa_K415001 Version 1 (Component)
P_const(low/med) : LuxR : Term : pLux/CI-OR : RBS-mCherry : Term

BBa_K750115
BBa_K750115 Version 1 (Component)
pBADcIT-PtetcIT-PcIGLT: arabinose or atc->cI protein->reduction of gfp(lva)

BBa_K2123112
BBa_K2123112 Version 1 (Component)
Tac promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)

BBa_K726009
BBa_K726009 Version 1 (Component)
T7 driven lac operated inducer for the rhl quorum-sensing system

BBa_I718001
BBa_I718001 Version 1 (Component)
ech My new test generator part Feruloyl CoA hydratase for vanilin

BBa_K648130
BBa_K648130 Version 1 (Component)
Pr of Or tester with cI repressor with very weak RBS

BBa_K648129
BBa_K648129 Version 1 (Component)
Pr of Or tester with cI repressor with slightly weak RBS

GFP-AID
BBa_K812110 Version 1 (Component)
GFP fusion with the ubuquitinase E3 OsTirI recoginition domain for PSC2+ plasmid

BBa_K750112
BBa_K750112 Version 1 (Component)
GFP(lva) expression system controlled by cI or atc(RBS strength:1.0)

BBa_K2020051
BBa_K2020051 Version 1 (Component)
wild type tyrosyl synthetase for use in E.coli with amber anticodon and Y32G

BBa_K2082001
BBa_K2082001 Version 1 (Component)
Nanobody: Constant regions (insert variable regions to create your defined Nanobody or library)

BBa_K415008
BBa_K415008 Version 1 (Component)
pLux/cI-OR : RBS-mCherry : Term : p(lac) IQ : luxR : Term : pLux

BBa_K415009
BBa_K415009 Version 1 (Component)
pLux/cI-OR : RBS-mCherry : Term : P(tetR) : RBS-luxR : Term : pLux

BBa_K1113411
BBa_K1113411 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome

BBa_I20292
BBa_I20292 Version 1 (Component)
There is no limit to what a man can do or where he can go if...

BBa_K2123116
BBa_K2123116 Version 1 (Component)
Universal promoter for both phase of growth in tandem with downstram mer operator + RFP (K081014)

BBa_J85010
BBa_J85010 Version 1 (Component)
PLux/cI-OR Promoter

Adapter Bi
BBa_K1807015 Version 1 (Component)
This device allows for the IPTG-inducible expression of lacZ&#945; peptide which in the presence of

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_I733007
BBa_I733007 Version 1 (Component)
Weight cells either turn blue or die in response to both inputs and HSL level

BBa_I741109
BBa_I741109 Version 1 (Component)
Lambda Or operator region

BBa_K549004
BBa_K549004 Version 1 (Component)
LacI promotor fused with the iron dependent regulator fur

BBa_K228013
BBa_K228013 Version 1 (Component)
(pSal PO) OR Gate - GFP

BBa_K415011
BBa_K415011 Version 1 (Component)
PtetR : RBS : LuxR : Term : PluxR/cI-OR : RBS : mCherry : Term : Plux/cI-OR : RBS : LuxI

BBa_K415005
BBa_K415005 Version 1 (Component)
pLux/cI-OR : RBS-mCherry : Term : p(tetR) : RBS-luxR : Term