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BBa_I2028

BBa_I2028 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I2028

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sara Neves (Fighting Darwins)

Date created: 2004-01-28 12:00:00

Date modified: 2015-08-31 04:07:40

RhlR Receiver Test (R1075.B0034.C0071.B0015.R0071.E0022)

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Details

Types	DnaRegion
Roles	engineered_region Project
Sequences	BBa_I2028_sequence (Version 1)

Description

rhlR receiver test:
-to test basal activity, give no input of chemical signal, and reporter indicates TIPs leakage

-to test binding, put in solution of chemical signal made by rhlI (AI-2), the inducer binds to the lasR protein to activate the promoter R0071, to make TIPs that will express reporter

Components

Access	Instance	Definition
public public public public public public public	BBa_R1075 BBa_B0034 BBa_C0071 BBa_B0010 BBa_B0012 BBa_R0071 BBa_E0022	const. BBa_B0034 rhlR BBa_B0010 BBa_B0012 RhlR+C4 ECFP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R1075 BBa_B0034 BBa_C0071 BBa_B0010 BBa_B0012 BBa_R0071 BBa_E0022	1,49 58,69 76,837 871,950 959,999 1008,1060 1067,1828	const. BBa_B0034 rhlR BBa_B0010 BBa_B0012 RhlR+C4 ECFP