BBa_I715048

BBa_I715048 Version 1

Component

This part has been discontinued.

Please consider using BBa_I715047 as a replacement.

Source:
http://parts.igem.org/Part:BBa_I715048
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Amanda Valencia
Date created: 2007-10-23 11:00:00
Date modified: 2015-08-31 04:07:50

Hin + pT7 -> RFP1 + GFP1 medium initial orientation



    • Details

    Types
    DnaRegion

    Roles
    Device

    engineered_region

    Sequences BBa_I715048_sequence (Version 1)

    Description

    Part is composed of a Hin recombinase expression casette driven by pLac and series of flippable units that include halves of GFP and RFP: T7prom-RBS-RFP1-hixC-RFP2-RBS-GFP1-hixC-RFP2-TT-hixC-GFP2-TT-hixC . The corresponding part without Hin glows redas a result of the expression of the hix-interrupted RFP. With Hin, most colonies are red, but green colonies appear. Evidence suggests that this is a result of Hin-mediated recombination.

    Notes

    None

    Source

    Ligation of existing part

    igem#experience
    None
     
    igem#sampleStatus
    Discontinued
    igem#status
    Deleted
     
    isReplacedBy
    BBa_I715047/1
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_I715048/1
     

    Attachments

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