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BBa_I724003

BBa_I724003 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_I724003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Susan Westfall

Date created: 2007-10-25 11:00:00

Date modified: 2015-08-31 04:07:54

Constitutive LuxR Generator + LacI regulated RFP(+LVA) and LuxI(LVA)

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_I724003_sequence (Version 1)

Description

This is a modification of the I15004 brick which creates LuxR constitutively rather than under a pTet promoter along with a RFP reporter regulated under the LacI promoter.

Notes

We wanted to establish a functional LuxI regulated by LacI with a fluorescent reporter which we chose as RFP.

Source

The I15004 plus modifications

Components

Access	Instance	Definition
public public public public public public public public public	BBa_R0011 BBa_B0034 BBa_C0061 BBa_J04051 BBa_B0010 BBa_J23119 BBa_B0034 BBa_C0062 BBa_B0010	lacI+pL BBa_B0034 luxI BBa_J04051 BBa_B0010 BBa_J23119 BBa_B0034 luxr BBa_B0010

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0011 BBa_B0034 BBa_C0061 BBa_J04051 BBa_B0010 BBa_J23119 BBa_B0034 BBa_C0062 BBa_B0010	1,54 64,75 82,699 731,1450 1459,1538 1547,1581 1590,1601 1608,2363 2397,2476	lacI+pL BBa_B0034 luxI BBa_J04051 BBa_B0010 BBa_J23119 BBa_B0034 luxr BBa_B0010

Other Properties

igem#experience	None
igem#sampleStatus	Discontinued
igem#status	Deleted
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_I724003/1