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BBa_I728979

BBa_I728979 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I728979

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bernice Huang

Date created: 2007-10-22 11:00:00

Date modified: 2015-08-31 04:07:55

I14032+B0034+BBa_I728000+B0014

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_I728979_sequence (Version 1)

Description

This is in improvement on part Bba_I728978. F2620 was replaced with a constitutive promoter and a double terminator stop was added after the CPX protein.

Notes

After obtaining extra unexpected bands on a western blot test of bacteria transformed with Bba_I1728978, we decided to add a double terminator to see if there was leaky transcription into the vector backbone.

Source

Bba_I728978

Components

Access	Instance	Definition
public public public public public	BBa_I14032 BBa_B0034 BBa_I728500 BBa_B0012 BBa_B0011	P(Lac) IQ BBa_B0034 CPX BBa_B0012 BBa_B0011

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I14032 BBa_B0034 BBa_I728500 BBa_B0012 BBa_B0011	1,37 46,57 64,717 726,766 775,820	P(Lac) IQ BBa_B0034 CPX BBa_B0012 BBa_B0011

Other Properties

igem#experience	Works
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_I728979/1