BBa_I730013

BBa_I730013 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I730013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Arnau Montagud Aquino

Date created: 2007-10-10 11:00:00

Date modified: 2015-08-31 04:07:55

pOmpRm-pLacI-TetR repressor-CFP

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_I730013_sequence (Version 1)

Description

pOmpRm-ptetR-strong RBS-LacI repressor(LVA+)-strong RBS-YFP-terminator

R0083.R0011.B0034.C0040.E0422

intermediate part for the construction of our 'comparator'. note that it bears no terminator sequence, as we aim to add a fluorescence protein as multicistronic parts.

it has the osmolarity-sensing mutated promoter for the OmpC gene, as the input of the device. this mutant is said to (and characterised as) be weaker than regular pOmpR

as an output we have thought of the fluorescence protein CFP.

Notes

N/A

Source

Registry
Valencia iGEM 2006 team project

Components

Access	Instance	Definition
public	BBa_R0083	OmpR
public	BBa_R0011	lacI+pL
public	BBa_B0034	BBa_B0034
public	BBa_C0040	tetR
public	BBa_E0422	BBa_E0422

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0083	1,78	OmpR
BBa_R0011	87,140	lacI+pL
BBa_B0034	150,161	BBa_B0034
BBa_C0040	168,852	tetR
BBa_E0422	861,1777	BBa_E0422

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I730013/1

Member of these Collections

• iGEM Parts Registry