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lacITst

BBa_J05505 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J05505

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: ETH Zurich iGEM Team 2005

Date created: 2005-09-21 11:00:00

Date modified: 2015-08-31 04:08:16

Lambda-lacI input system TESTER (IPTG controlled lacI+pL efficiency)

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Details

Types	DnaRegion
Roles	engineered_region Measurement
Sequences	BBa_J05505_sequence (Version 1)

Description

Constitutive (tetR) lacI-production (lacI+LVA) to block promoter (lacI+pL) in ground state of a 2-state system. lacI+pL can be activated, e.g. over IPTG, thus leading to the production of GFP (green fluorescence). This device allows to estimate repressed (lacI) activity/full activity under ITPG absence/presence.

Notes

For more details see Sensing Device and Event Processing Device documentation.

Source

Registry 7.05

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_R0040 BBa_B0034 BBa_C0012 BBa_B0010 BBa_B0012 BBa_R0011 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012	p(tetR) BBa_B0034 lacI BBa_B0010 BBa_B0012 lacI+pL BBa_B0034 GFP BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040 BBa_B0034 BBa_C0012 BBa_B0010 BBa_B0012 BBa_R0011 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012	1,54 63,74 81,1208 1242,1321 1330,1370 1379,1432 1442,1453 1460,2179 2188,2267 2276,2316	p(tetR) BBa_B0034 lacI BBa_B0010 BBa_B0012 lacI+pL BBa_B0034 GFP BBa_B0010 BBa_B0012

Other Properties

igem#experience	None
igem#sampleStatus	Not in stock
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_J05505/1