Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Davidson and Missouri Western
Date created: 2006-07-31 11:00:00
Date modified: 2015-08-31 04:08:45
pBad-HixC-RBS-TetF-HixC-TT-RE
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_J3103_sequence (Version 1) |
Description
This part contains the coding region for tetracylcine resistance flanked by Hix C sites. This part is going to be used to test the optimal location of the RBS. If the Hin(BBa_J31000) or Hin LVA (BBa_J31001) genes are placed upstream of this part there is a possibility that the coding region between the Hix C sites is inverted.Notes
This part is going to be used to test where the RBS needs to be placed.Source
None| Access | Instance | Definition |
| public public public public public public public public | BBa_I13453 BBa_J44000 BBa_B0030 BBa_J31007 BBa_J44000 BBa_B0010 BBa_B0012 BBa_J3101 | BBa_I13453 hixC BBa_B0030 tetA(C)f hixC BBa_B0010 BBa_B0012 RE |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I13453 BBa_J44000 BBa_B0030 BBa_J31007 BBa_J44000 BBa_B0010 BBa_B0012 BBa_J3101 | 1,130 139,164 173,187 194,1384 1393,1418 1427,1506 1515,1555 1564,1640 | BBa_I13453 hixC BBa_B0030 tetA(C)f hixC BBa_B0010 BBa_B0012 RE |