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BBa_J32001

BBa_J32001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J32001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Austen Heinz

Date created: 2006-07-10 11:00:00

Date modified: 2015-08-31 04:08:45

Targeted Localization

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Details

Types	DnaRegion
Roles	Composite engineered_region
Sequences	BBa_J32001_sequence (Version 1)

Description

Neisseria gonorrhoeae IgA Protease antibody display.

Notes

It is a fusion protein so biobrick prefixes and suffixes have been modified to 10 and 24 bp, removing the bp between the flanking restriction sites and the sequence. Otherwise it would read out of frame with 8bp overlap

Source

The signal sequence PelB and S*Tag sequence came from Novagen. The antibody sequence is for cAb-CEA5 nanobody from Ablynx. The C-terminus of Neisseria gonorrhoeae IgA Protease NCBI accession number AE004969

Components

Access	Instance	Definition
public public public public public public public public public public public public public	BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012 BBa_R0040 BBa_B0034 BBa_J32015 BBa_J32016 BBa_J32017 BBa_J32018 BBa_B0010 BBa_B0012	p(tetR) BBa_B0034 GFP BBa_B0010 BBa_B0012 p(tetR) BBa_B0034 PelB AntiCEA S*Tag C-IgAP BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012 BBa_R0040 BBa_B0034 BBa_J32015 BBa_J32016 BBa_J32017 BBa_J32018 BBa_B0010 BBa_B0012	1,54 63,74 81,800 809,888 897,937 946,999 1008,1019 1026,1091 1100,1486 1495,1540 1549,2805 2814,2893 2902,2942	p(tetR) BBa_B0034 GFP BBa_B0010 BBa_B0012 p(tetR) BBa_B0034 PelB AntiCEA S*Tag C-IgAP BBa_B0010 BBa_B0012

Other Properties

igem#experience	None
igem#sampleStatus	Not in stock
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_J32001/1