BBa_J44008

BBa_J44008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J44008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Adam Brown

Date created: 2006-08-21 11:00:00

Date modified: 2015-08-31 04:08:49

pLac-RBS-HinLVA-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_J44008_sequence (Version 1)

Description

This a device that uses Hin to produce Hin recombinase that can flip the promotor pBADrev for Tet resistance; this device can turn on/off the genes for Tet resistance. It uses HinLVA as a degradation tag, which will terminate the flipping of the HixC when the HinLVA is completely degraded.

Notes

Possibility of read-thru of the pBADrev during transcription.

Source

(BBa_B0030), (BBa_B0015), (BBa_R0010), (BBa_J31007), (BBa_J3101)- from registry. (BBa_J31001) - from Davidson College. (BBa_J44000) - made in collaboration with Missouri Western/Davidson. (BBa_J44002) - from Missouri Western State University.

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_B0030	BBa_B0030
public	BBa_J31001	Hin LVA
public	BBa_B0015	BBa_B0015
public	BBa_J44000	hixC
public	BBa_J44002	BBa_J44002
public	BBa_J44000	hixC
public	BBa_B0030	BBa_B0030
public	BBa_J31007	tetA(C)f
public	BBa_B0015	BBa_B0015
public	BBa_J3101	RE

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_B0030	209,223	BBa_B0030
BBa_J31001	230,841	Hin LVA
BBa_B0015	850,978	BBa_B0015
BBa_J44000	987,1012	hixC
BBa_J44002	1021,1150	BBa_J44002
BBa_J44000	1159,1184	hixC
BBa_B0030	1193,1207	BBa_B0030
BBa_J31007	1214,2404	tetA(C)f
BBa_B0015	2413,2541	BBa_B0015
BBa_J3101	2550,2626	RE

Other Properties

• igem#experience: Issues
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J44008/1

Member of these Collections

• iGEM Parts Registry