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BBa_K079027

BBa_K079027 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K079027

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Francesca Ceroni

Date created: 2008-10-23 11:00:00

Date modified: 2015-05-08 01:08:33

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Details

Types	DnaRegion
Roles	Reporter engineered_region
Sequences	BBa_K079027_sequence (Version 1)

Description

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Notes

We wanted to characterize the Lac promoter repression by different levels of LacI protein. Thus, we cloned the BBa_S0100 part under the control of three promoters from the Berkley costitutive promoter librarywith different transcriptional strenght: BBa_J23114, BBa_J23105and BBa_J23118. As a reporter, the GFP protein (BBA_I763020) was cloned downstream of the Lac promoter (BBa_J04500).

Source

Registry standard part

Components

Access	Instance	Definition
public public public public public public public public public public public	BBa_J23114 BBa_B0034 BBa_C0012 BBa_B0010 BBa_B0012 BBa_R0010 BBa_B0034 BBa_B0034 BBa_J04031 BBa_B0010 BBa_B0012	BBa_J23114 BBa_B0034 lacI BBa_B0010 BBa_B0012 LacI BBa_B0034 BBa_B0034 BBa_J04031 BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23114 BBa_B0034 BBa_C0012 BBa_B0010 BBa_B0012 BBa_R0010 BBa_B0034 BBa_B0034 BBa_J04031 BBa_B0010 BBa_B0012	1,35 44,55 62,1189 1223,1302 1311,1351 1360,1559 1568,1579 1588,1599 1606,2364 2373,2452 2461,2501	BBa_J23114 BBa_B0034 lacI BBa_B0010 BBa_B0012 LacI BBa_B0034 BBa_B0034 BBa_J04031 BBa_B0010 BBa_B0012

Other Properties

igem#experience	Works
igem#sampleStatus	Discontinued
igem#status	Deleted
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K079027/1