BBa_K081022

BBa_K081022 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K081022

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lorenzo Pasotti, Paolo Magni

Date created: 2008-10-18 11:00:00

Date modified: 2015-05-08 01:08:35

Plambda regulated luxR generator and Plux

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K081022_sequence (Version 1)

Description

This device produces luxR constitutively under the strong promoter Plambda, in absence of cI repressor. Plambda can be turned off by cI repressor and luxR production is inhibited.

This composite part can be considered as 3OC6-HSL in -> PoPS out device, because 3OC6-HSL binds luxR which turns on Plux promoter. The output PoPS can also be regulated by cI repressor, which modulates luxR expression.

Strong RBS (efficiency=0.6).

Artificial 39 bp terminator (efficiency=0.99).
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Notes

We used BioBrick Standard Assembly.

Source

Plambda:
RBS:
luxR:
T:

Components

Access	Instance	Definition
public	BBa_R0051	cI lam
public	BBa_B0030	BBa_B0030
public	BBa_C0062	luxr
public	BBa_B1006	BBa_B1006
public	BBa_R0062	lux pR

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0051	1,49	cI lam
BBa_B0030	58,72	BBa_B0030
BBa_C0062	79,834	luxr
BBa_B1006	868,906	BBa_B1006
BBa_R0062	915,969	lux pR

Other Properties

• igem#experience: Works
• igem#partStatus: Released HQ 2013
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K081022/1

Member of these Collections

• iGEM Parts Registry