Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sven Jager, Sebastian Wagner and Anne Schieferdecker
Date created: 2013-09-17 11:00:00
Date modified: 2015-05-08 01:08:57
FsC: functional cutinase
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1055007_sequence (Version 1) |
Description
The part enables the user to produce functional cutinase from fungus Fusarium solani pisi which is a cuticula degrading hydrolase. The enzyme is fused to mRFP to allow tracking of expression and also provides a His-Tag for subsequent purification. Both, mRFP and His-Tag, can be cleaved using TEV-Protease.Notes
Despite BioBricks the part also consists of the RBS from pET22b(+), a TEV-site and a His-Tag.Source
fungus Fusarium solani pisi| Access | Instance | Definition |
| public public public public | BBa_K206000 BBa_J32015 BBa_K808025 BBa_E1010 | pBAD PelB BBa_K808025 mRFP1 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K206000 BBa_J32015 BBa_K808025 BBa_E1010 | 1,130 137,202 209,859 866,1571 | pBAD PelB BBa_K808025 mRFP1 |