Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Naoki Watarai
Date created: 2013-09-08 11:00:00
Date modified: 2015-05-08 01:09:22
Plux-M13-Plac-GFP on pSB3
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1139021_sequence (Version 1) |
Description
M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell.This biobrick is derived from M13mp18 phage vector by PCR. It inclueds 11 ORFs, M13 origin, a packaging sequence and Lac promoter. The promoter on the upstream of g2(gene 2) is altered to Lux promoter.
A phage particle is formed only when the host cell receives AHL signal(3OC6HSL, C6) because g2p(gene 2 protein) is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle.
As a reporter, GFP is inserted on the downstream of the Lac promoter.
Notes
TBDSource
Lux promoter from oligo DNA,M13 genes and origin from BBa_K1139019,
GFP from BBa_E0040,
rbs from BBa_B0030,
double terminator from BBa_B0015
are combined.