| Types | DnaRegion
|
| Roles | engineered_region
Composite
|
| Sequences | BBa_K1157021_sequence (Version 1)
|
Description
This is a biosensor for C-4 AHL molecules(a type of quorum sensing(QS) molecule) produced by Psuedomonas aeruginosa. We use a constitutive promoter to express Rhl receptor, and when C-4 AHL binds with Rhl receptor(RhlR), it will combine with its specific promoter sequence pRhl and regulate the expression of producing more RhlR and GFP. By sensing the GFP signal, we are able to detect QS signals.
Notes
There are detailed design of the sequence, and data has shown that with the reporter placed BEFORE the constitutive promoter, better functional results can be yield.
Source
It is built by the original biobricks produced by iGEM oficial.
