BBa_K116504

BBa_K116504 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K116504

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chun-Ju Yang

Date created: 2008-10-28 12:00:00

Date modified: 2015-05-08 01:09:33

Tet+RBS+KaiA+Ter+Lac+RBS+KaiB+Lac+RBS+KaiC

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K116504_sequence (Version 1)

Description

KaiA and KaiBC is regulated by different promoter in order to generate different amount of Kai proteins at a ratio similar to that measured in vivo KaiA:KaiB:KaiC=1:1:4 (by weight) 1:2.8:2.2 (by amount)

Notes

1. M. Nakajima et al., Science 308, 414 (Apr 15, 2005).

Source

BBa_R0040 BBa_B0034 BBa_J36801 BBa_B0015 BBa_J36336

Components

Access	Instance	Definition
public	BBa_R0040	p(tetR)
public	BBa_B0034	BBa_B0034
public	BBa_J36801	KaiA
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012
public	BBa_R0010	LacI
public	BBa_B0034	BBa_B0034
public	BBa_J36802	KaiB
public	BBa_R0010	LacI
public	BBa_B0034	BBa_B0034
public	BBa_J36804	KaiC

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040	1,54	p(tetR)
BBa_B0034	63,74	BBa_B0034
BBa_J36801	83,940	KaiA
BBa_B0010	949,1028	BBa_B0010
BBa_B0012	1037,1077	BBa_B0012
BBa_R0010	1086,1285	LacI
BBa_B0034	1294,1305	BBa_B0034
BBa_J36802	1312,1623	KaiB
BBa_R0010	1632,1831	LacI
BBa_B0034	1840,1851	BBa_B0034
BBa_J36804	1858,3420	KaiC

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K116504/1

Member of these Collections

• iGEM Parts Registry