Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Katty Wu
Date created: 2014-06-15 11:00:00
Date modified: 2015-05-08 01:09:46
pTet promoter + Lock (crRNA) + ccdB + dterm
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1300001_sequence (Version 1) |
Description
pTet is a constitutive promoter, meaning that the Cis+ccdB gene is continuously transcribed; however, because the Cis portion of the mRNA forms a hairpin which blocks the RBS, the gene cannot be translated. In this way, the Cis acts as a "lock" which will only allow translation, and therefore cell death, once opened. We designed a separate gene to act as a "key," controlled by a UV-light-inducible promoter (BBa_K1300000).Notes
We took the pTet+Cis out of the Registry and added a toxin behind it in order to test the riboregulatory system, by means of a kill switch.Source
The components for this part were obtained from the iGEM Registry of Standard Biological Parts.| Access | Instance | Definition |
| public public public | BBa_J01060 BBa_K414005 BBa_B0015 | [pTET][loc BBa_K414005 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J01060 BBa_K414005 BBa_B0015 | 1,102 111,770 779,907 | [pTET][loc BBa_K414005 BBa_B0015 |