BBa_K136040

BBa_K136040 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K136040

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: alexandra bouaziz

Date created: 2008-10-24 11:00:00

Date modified: 2015-05-08 01:10:03

pFlgA-RFP

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K136040_sequence (Version 1)

Description

Promoter of flgA gene from E. coli flagella

flgA is a class 2 gene that is part of the construction of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ).

Its promoter is consitutively repressed.

It is activated by FlhD4C2 and maybe by FliA with different strength ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).

flgA promotor is here followed by RFP.

Notes

pFlgA was digested by EcoRI ,SpeI (FI )and cloned into the J61002 plasmid containing RFP (BV).

Source

flgA promotor comes from E. coli K12 strain MG1655.

Components

Access	Instance	Definition
public	BBa_K136006	BBa_K136006
public	BBa_J61002	BBa_J61002

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K136006	1,202	BBa_K136006
BBa_J61002	211,3158	BBa_J61002

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K136040/1

Member of these Collections

• iGEM Parts Registry