Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Exeter iGEM 2014
Date created: 2014-08-10 11:00:00
Date modified: 2015-05-08 01:10:15
NemA (Inducible Construct)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1398003_sequence (Version 1) |
Description
A construct created to degraded TNT and nitroglycerine.The construct contains the coding sequence for NemA (BBa_K1398002), an enzyme involved in the degradation of toxic compounds for their reuse in nitrogen metabolism.
The construct also contains an inducible promoter (BBa_R0010), a strong RBS (BBa_B0034) and a double terminator (BBa_B0010).
The protein has been codon-optimised for expression in E. coli.
Notes
This construct was created to allow for the over-expression of NemA, so it contains high expression regulatory sequences. A lactose inducible promoter has been used to give control over expression.Source
The original source of NemA was E. coli. The regulatory sequences were acquired from the iGEM repository.| Access | Instance | Definition |
| public public public public | BBa_R0010 BBa_B0034 BBa_K1398002 BBa_B0015 | LacI BBa_B0034 BBa_K1398002 BBa_B0015 |