BBa_K145205

BBa_K145205 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K145205

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jonas Demeulemeester

Date created: 2008-08-19 11:00:00

Date modified: 2015-05-08 01:10:29

OUTPUT GFP under TetR pomoter

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K145205_sequence (Version 1)

Description

OUTPUT GFP with an LVA tag under the control of the OmpF pomoter, which is activated by a low concentration and repressed by a high concentration of phosphorylated OmpR.

Notes

When light hits the lightsensor M30109 (K145000). It's kinase activity will be inhibited and only a small amount of OmpR-P is produced. This means the OmpF promoter will be actively used for transcription.

Source

other parts

Components

Access	Instance	Definition
public	BBa_R0040	p(tetR)
public	BBa_B0032	BBa_B0032
public	BBa_K145015	GFP
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040	1,54	p(tetR)
BBa_B0032	63,75	BBa_B0032
BBa_K145015	82,840	GFP
BBa_B0010	849,928	BBa_B0010
BBa_B0012	937,977	BBa_B0012

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K145205/1

Member of these Collections

• iGEM Parts Registry