Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Choi hiu Ying, LEE Cheuk Lam, Tse Yui Shing, Yeung Sin Yu
Date created: 2014-09-27 11:00:00
Date modified: 2015-05-08 01:10:39
IPTG inducible expression of ∆
9, ∆
12, and ∆
15 desaturases
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1472611_sequence (Version 1) |
Description
It is a device that contained a promoter (BBa_R0010), RBS (BBa_B0034) for each desaturase gene ∆9,12 and ∆15. When free fatty acids, for example, stearic acid (18:0) enters cell, it is converted into oleic acid (18:1∆9) by ∆9 desaturase, then to linolenic acid (18:2∆9,12) by ∆12 desaturase and finally to alpha-Linolenic acid, ALA (18:3∆9,12,15) by ∆15 desaturase. This device is designated to express under the presence of IPTG.Notes
The size of the whole device could be relatively large. The selection of promoter is important as it controls when the machine starts producing desaturases. The investment of producing 3 enzymes in a time could be a energy burden for E. coli.Source
The amino acid sequences of desaturases genes are derived from Synechocystis sp., but codon optimised for expression in E. coli.| Access | Instance | Definition |
| public public public public public public public | BBa_R0010 BBa_B0034 BBa_K1027001 BBa_B0034 BBa_K1027002 BBa_B0034 BBa_K1472610 | LacI BBa_B0034 BBa_K1027001 BBa_B0034 BBa_K1027002 BBa_B0034 BBa_K1472610 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 BBa_B0034 BBa_K1027001 BBa_B0034 BBa_K1027002 BBa_B0034 BBa_K1472610 | 1,200 209,220 227,1186 1195,1206 1213,2274 2283,2294 2301,3384 | LacI BBa_B0034 BBa_K1027001 BBa_B0034 BBa_K1027002 BBa_B0034 BBa_K1472610 |