Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Iowis Zhu
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:44
pBAD-RBS-Bovine Galectin-1-B0012 Terminator
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1489000_sequence (Version 1) |
Description
Soluble beta-galactoside binding protein (galectin) originally from Bos taurus. Believed to expressed both intracellularly and extracellularly, where they serve to binding various types of beta-galactosides and initialize signal cascades. Believed to be a dimer in its active form, as is true with most mammalian galectins.This biobrick is under the pBAD promoter (BBa_K206000) with attached RBS (BBa_B0034), and utilizes an additional RNA polymerase terminator (BBa_B0012).
UMaryland 2014 is interested in utilizing this part to investigate whether E. coli can recognize and bind to surface carbohydrates on a marine pathogen. Other uses of this part lie in the recognition of various carbohydrate ligands and potential activation of signal transduction pathways.
Notes
EcoRI site in middle of Bovine Galectin gene was removed, codons were optimized for expression in E. coli.Source
Sequence generated from Bos taurus genome, reoptimized for E. coli expression.| Access | Instance | Definition |
| public public public public | BBa_K206000 BBa_B0034 BBa_K1489001 BBa_B0012 | pBAD BBa_B0034 BtGal1 BBa_B0012 |