BBa_K1532005

BBa_K1532005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1532005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Xinyuan Qiu

Date created: 2014-09-29 11:00:00

Date modified: 2015-05-08 01:10:52

Pbad->LuxR

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K1532005_sequence (Version 1)

Description

This part is a functional device with the input of AraC & arabinose and the out put of the protein LuxR. The expression of the output signal will be activated if the protein AraC and the small molecule arabinose exists the same time. This part may not work when the glucose exists in the culture.

Notes

The BBa_R0080 we used in the device contains a cup-site so that the glucose may affect the work of this device. So, glucose-free culture is suggested to be used if you want to use this device.

Source

This part is assembled with standard bio-bricks from the distribution with the standard assembly protocol.

Components

Access	Instance	Definition
public	BBa_K1532000	BBa_K1532000
public	BBa_R0080	AraC
public	BBa_B0030	BBa_B0030
public	BBa_C0062	luxr
public	BBa_B0015	BBa_B0015
public	BBa_K1532000	BBa_K1532000

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1532000	1,10	BBa_K1532000
BBa_R0080	19,167	AraC
BBa_B0030	176,190	BBa_B0030
BBa_C0062	197,952	luxr
BBa_B0015	986,1114	BBa_B0015
BBa_K1532000	1123,1132	BBa_K1532000

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1532005/1

Member of these Collections

• iGEM Parts Registry