Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stefan Robert Marsden
Date created: 2015-09-03 11:00:00
Date modified: 2015-09-18 03:37:06
pRha + Mfp5_CsgA_His fusion protein
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1583104_sequence (Version 1) |
Description
CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms taken from E.coli K-12 MG1655. Inspired by mussels, the Mfp5 (mussel foot protein) has high adhesive properties towards wet polar surfaces.By creating a fusion protein, the adhesive properties of the mussel foot protein is combined with the formation of nanowires.
This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1]
1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
The CsgA sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).
Notes
A mutation had to be introduced at base pair 766 to remove a PstI restriction site. The protein sequence was not changed in doing so.Source
This part originates from the E.coli K-12 MG1655 genome and from mussels.The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1]
1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
| Access | Instance | Definition |
| public public public public public public public public public | BBa_K1583054 BBa_K914003 BBa_K1583056 BBa_K1583061 BBa_K1583002 BBa_K1583012 BBa_K1583013 BBa_K1583059 BBa_K1583057 | BBa_K1583054 BBa_K914003 BBa_K1583056 BBa_K1583061 BBa_K1583002 BBa_K1583012 BBa_K1583013 BBa_K1583059 BBa_K1583057 |